品名： 普诺赛NCI-H146 [H146] (人小细胞肺癌细胞)

型号： CL-0693 产品详情 金山科研平台现货促销

产品信息

NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确)
	H146; H-146; NCIH146
	Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected.
	人类
	男性，59岁
	肺
	悬浮细胞
	淋巴母细胞样
	肿瘤细胞
	肺癌细胞
	RPMI-1640＋10% FBS＋1% P/S
	1:2-1:3
	2~3次/周
	冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮
	气相：空气，95%；CO2，5%温度：37℃
	vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes -
	该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。
	ATCC; HTB-173

STR鉴定

STR位点信息

Amelogenin	X
CSF1PO	11
D2S1338	19
D3S1358	11
D5S818	12
D7S820	9,10
D8S1179	12,14
D13S317	12
D16S539	11
D18S51	15,17
D19S433	13,14

D21S11	30
FGA	23
PentaD	13
PentaE	12
TH01	6,9.3
TPOX	8,11
vWA	14,16
D6S1043	11,19
D12S391	18.3,25
D2S441	10,14

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