普诺赛M-07e (人巨细胞白血病细胞)

2024-07-20

品名: 普诺赛M-07e (人巨细胞白血病细胞)

型号: CL-0686 产品详情 金山科研平台现货促销

产品信息

M-07e (人巨细胞白血病细胞) (STR鉴定正确)
M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E
Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available
人类
女性,6个月
外周血
悬浮细胞
淋巴母细胞样
肿瘤细胞
白血病细胞
1
RPMI-1640+10% FBS+8ng/mL GM-CSF+1% P/S
5×10^5-1×10^6cells/mL
2~3次/周
~40小时
冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
气相:空气,95%;CO2,5%温度:37℃
CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。
DSMZ;ACC-104
STR鉴定

STR位点信息

AmelogeninX
CSF1PO9,10
D2S133819,25
D3S135816,19
D5S81811
D7S82011
D8S117913,16
D13S31710,11
D16S53911
D18S5115,20
D19S43314,15
D21S1130,32.2
FGA18,22
PentaD9
PentaE12,14
TH016,8
TPOX8
vWA16,18
D6S104313,19
D12S39123,24
D2S44114

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