品名： 普诺赛HT-29 (人结肠癌细胞)

型号： CL-0118 产品详情 金山科研平台现货促销

产品信息

HT-29 (人结肠癌细胞) (STR鉴定正确)
	HT 29; HT29
	HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的；近来，已建株的培养细胞用含相同血清McCoy's5A培液培养。HT-29细胞在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。
	人
	女；44岁
	结直肠腺癌
	贴壁细胞
	上皮细胞样
	肿瘤细胞
	肠癌细胞
	1
	McCoy’s 5A＋10% FBS＋1% P/S
	1:3-1:4
	2~3次/周
	~36-60 hours
	冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮
	气相：空气，95%；CO2，5%温度：37℃
	Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters.
	human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A
	secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin, myc+; ras+; myb+; fos+; sis+; p53+; abl -; ros -; src -, Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5, HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV).
	ATCC; HTB-38 BCRJ; 0111 CCLV; CCLV-RIE 0960 DSMZ; ACC-299 ECACC; 91072201

STR鉴定

STR位点信息

Amelogenin	X
CSF1PO	11,12
D2S1338	19,23
D3S1358	15,17
D5S818	11,12
D7S820	10
D8S1179	10,16
D13S317	11,12
D16S539	11,12
D18S51	13
D19S433	14

D21S11	29,30
FGA	20,22
PentaD	11,13
PentaE	14,16
TH01	6,9
TPOX	8,9
vWA	17,19
D6S1043	12,14
D12S391	18.3,21
D2S441	11

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