品名： Brilliant Violet 421™ anti-mouse CD4 Antibody

型号： 100443 产品详情 金山科研平台现货促销

Product Details

Isotype Control

Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CTL clone V4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality testedICC- Verified

SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4⁺ T cell activation^1,4,11, thymocyte costimulation³, in vitro and in vivo depletion^2,5-8, blocking of egg-sperm cell adhesion^1,4, immunohistochemical staining of acetone-fixed frozen sections^9,10, immunoprecipitation^1,2, and spatial biology (IBEX)^12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation.Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References(PubMed link indicates BioLegend citation)

See More

Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)

Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)

Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)

Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)

Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)

Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)

Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)

Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)

Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)

Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)

Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)

RadtkeAJ,et al.2020.Proc Natl Acad Sci U S A.117:33455-65. (SB)PubMed

Product Citations

See More

Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed

Xu J et al. 2018. Cell. 173(3):762-775 . PubMed

Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed

Goswami M, et al. 2019. Biomed Opt Express. 10:151. PubMed

Yue X, et al. 2019. Nat Commun. 10:2011. PubMed

Bennion BG, et al. 2019. J Virol. 93. PubMed

Silva DA, et al. 2019. Nature. 565:186. PubMed

Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed

Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed

Annu K, et al. 2020. Sci Rep. 10:2359. PubMed

Han Y, et al. 2020. Nat Commun. 11:1776. PubMed

Huai W, et al. 2019. J Exp Med. 216:772. PubMed

RRID

AB_10900241 (BioLegend Cat. No. 100437) AB_2562557 (BioLegend Cat. No. 100443) AB_11203718 (BioLegend Cat. No. 100438)

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