品名： DMEM, powder, high glucose, pyruvate

品牌： 赛默飞世尔

型号： 12800017 产品详情 金山科研平台现货促销

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• HEPES
• L-glutamine	• Sodium Bicarbonate
• Phenol Red
• Sodium Pyruvate

The completeformulationis available.Using DMEMDMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).cGMP manufacturing and quality systemDMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Glucose:	High Glucose
HEPES Buffer:	No HEPES
Product Line:	Gibco®
数量.:	10 x 1L
Serum Supplementation:	Standard Serum Supplementation
Shipping Condition:	Room Temperature
Sodium Bicarbonate Buffer:	No Sodium Bicarbonate
Sodium Pyruvate Additive:	Sodium Pyruvate
性状:	Powder
Glutamine:	L-Glutamine
Phenol Red Indicator:	Phenol Red

内容及储存

Storage conditions: 2-8° CShipping conditions: AmbientShelf life: 36 months from date of manufacture