BIO-RADReadyPrep™ 蛋白萃取试剂盒（细胞质/原子核）1632089

ReadyPrep™ Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps #1632089

概述

50 preps, includes 50 ml cytoplasmic protein extraction buffer (CPEB), 50 ml protein solubilization buffer, 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 50 mg of cells or tissue

Use the ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) to enrich for both cytoplasmic and nuclear proteins. The kit separates cytoplasmic proteins from intact nuclei and then solubilizes nuclei.

Employs a specially formulated buffer and differential centrifugation to isolate intact nuclei

Contains a strongly chaotropic extraction buffer to efficiently solubilize nuclear proteins

Includes the components of the ReadyPrep 2-D Cleanup Kit to remove extraction buffer that may interfere with IEF

Extracted proteins are suitable for IEF, SDS-PAGE, and western blotting

The ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) uses a buffer/differential centrifugation protocol to generate enriched cytoplasmic fractions and intact nuclei (Dignam et al. 1983, Zerivitz and Akusjarvi 1989). The nuclei are treated with a strongly chaotropic extraction buffer to efficiently solubilize the nuclear proteins. The cytoplasmic fraction is processed with the included ReadyPrep 2-D Cleanup Kit to remove IEF-inhibiting compounds.  Although this kit is directed towards the preparation of nuclear and cytoplasmic protein samples for 2-D gel electrophoresis, the fractions are suitable for SDS-PAGE, western blotting, and other applications.

Kit Components

For 50 extractions of 50 mg of cells or tissue:

Cytoplasmic protein extraction buffer (CPEB), 50 ml

Protein solubilization buffer (PSB), 25 g

3PSB diluent, 30 ml

ReadyPrep 2-D Cleanup Kit (1632130)

Instruction manual

Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.