iScript CDNA 合成试剂盒 用于两步法的 real-time RT-PCR

2022-04-14

Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.

Key Features and Benefits

·Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction

·Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions

·Fast 26 min cDNA synthesis protocol

· Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase


See the iScript cDNA Synthesis Kit product page for more information and documentation.

Related Products

·View all cDNA Synthesis Kits

·Real-Time PCR Supermixes and Kits

·PrimePCR™ PCR Primers & Assays

Related Categories

·PCR and qPCR Reagents

·Real-Time PCR Detection Systems

·PCR Plastic Consumables

·Instrument Validation Tools and Services

Supporting Documents

·iScript cDNA Synthesis Kit Product Insert

·Amplification Plastics and Reagents Selection Guide

Online Resources

·qPCR/Real-Time PCR

· Genomics


Number of 20 µl Reactions Supermix Volume, µl Catalog Number
   25   100 (1 x 100 µl) 1708890
   100   400 (1 x 400 µl) 1708891
   500   2,000 (5 x 400 µl) 1708891BUN


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