产品英文名称：RNA Polymerase II H5 Monoclonal Antibody

产品中文名称：RNA聚合酶II H5单克隆抗体

Covance RNA聚合酶II H5单克隆抗体 Polymerase II H5 Monoclonal Antibody英文介绍：

Description;：Monoclonal Antibody against RNA Polymerase II

Also Known As ：DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit

Intended Use：** **Research Use Only (RUO)**

This product is sold for laboratory research use only, not for human or in-vivo use. **

Clone ：H5

Form：Ascites

Host ：Mouse

Species： Reactivity Extensive (Yeast to Human)

IsoType ：IgM

Specificity ：The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 2 version of pol II.

Uses ：This antibody is effective in immunofluorescence (IF), immunoblotting (WB), immunoprecipitation (IP), pol II purification and functional studies.

Suggested Working Dilution ：Quality control tests were done by Western blot and Dot blot. The optimal working dilution should be determined for each specific assay condition.

WB: 1:500*

IF: 1:50 – 1:100

IP: 1:100

Notes：*Predicted MW = 220-240 kDa

*use casein or BSA in the blocking solution rather than non-fat-dry milk

Storage ：Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.

Covance RNA聚合酶II H5单克隆抗体使用注意事项：

建议工作稀释度 质量控制测试是由Western blot和Dot blot完成。最佳工作稀释度应为每个特定的实验条件来确定。

WB：1:500*

IF：1:50 – 1:100

IP：1:100

注: 预测分子量=220-240 kDa的

使用酪蛋白或BSA的封闭液，而不是非脂肪干乳。

储存：储存于-20°C。经初步解冻，分配到分装并储存于-20°C。但应避免反复冻融，以防止变性的抗体。对于长期储存，保持抗体在-80°C。