产品英文名称：CD4+CD25+ Regulatory T Cell Isolation Kit, mouse

产品中文名称：美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒

美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒产品英文描述：

The CD4+CD25+ Regulatory T Cell Isolation Kit was developed for the isolation of CD4+CD25+ regulatory mouse T cells from single-cell suspensions of spleen and lymph nodes. The isolation is performed in a two-step procedure.

CD4+CD25+ immunoregulatory T cells have been shown to actively suppress immune responses against autologous and foreign antigens in vivo and in vitro. CD25, the IL-2Rα chain, is also expressed on activated CD4+ T cells, CD8+ T cells, dendritic cells, and B cells.

Miltenyi 美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒研究背景：

The CD4+CD25+ Regulatory T Cell Isolation Kit was developed for the isolation of mouse CD4+CD25+ regulatory T cells from singlecell suspensions of spleen or lymph nodes.

CD4+CD25+ immunoregulatory T cells have been shown to actively suppress immune responses against autologous and foreign antigens in vivo and in vitro. CD25, the IL-2Rα chain, is also expressed on activated CD8+ T cells, dendritic cells (DCs), and B cells.

The kit contains a cocktail of lineage specific biotin-conjugated antibodies against CD8 (Ly-2), CD11b (Mac-1), CD45R (B220),CD49b (DX5), Ter-119, and Anti-Biotin MicroBeads for depletion of non-CD4+ T cells, as well as CD25-PE and Anti-PE MicroBeads for subsequent positive selection of CD4+CD25+ regulatory T cells.

美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒产品介绍：

Components

1 mL CD4+CD25+ Regulatory T Cell Biotin-Antibody Cocktail, mouse:

Cocktail of biotin-conjugated monoclonal

anti-mouse antibodies against:

CD8a (Ly-2; isotype: rat IgG2a),

CD11b (Mac-1; isotype: rat IgG2b),

CD45R (B220; isotype: rat IgG2a),

CD49b (DX5; isotype: rat IgM)

and Ter-119 (isotype: rat IgG2b).

2 mL Anti-Biotin MicroBeads:

MicroBeads conjugated to monoclonal antibiotin antibody (isotype: mouse IgG1)

1 mL CD25-PE, mouse:

Monoclonal anti-mouse CD25 antibody conjugated to R-Phycoerythrin (PE) (clone: 7D4;isotype: rat IgM)

1 mL Anti-PE MicroBeads:

MicroBeads conjugated to monoclonal anti-PE antibodies (isotype: mouse IgG1)

Size

For 109 leukocytes, up to 100 separations.

Product format All components are supplied in buffer containing stabilizer and 0.05% sodium azide.

Storage

Store protected from light at 2–8 °C. Do not freeze.

The expiration date is indicated on the vial label.

Miltenyi 美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒产品应用：

Isolation of CD4+CD25+ regulatory T cells from single-cell suspensions of spleen or lymph nodes for:

• co-culture experiments with DCs to study priming of DCs for tolerance induction in vitro and after adoptive transfer of primed DCs in vivo.

• adoptive transfer experiments (e.g. from UV-exposed mice) to analyze the role of regulatory T cells during induction and elicitation of hapten-specific tolerance.