产品英文名称：Miltenyi CD4+ T Cell Isolation Kit II, mouse

产品中文名称：美天旎小鼠CD4+ T细胞分离试剂盒II

Miltenyi 美天旎小鼠CD4+ T细胞分离试剂盒II英文描述：

PLEASE NOTE: The separation protocol is faster now! Download the updated datasheet.

The CD4+ T Cell Isolation Kit II was developed for the untouched isolation of CD4+ T cells from single-cell suspensions from mouse spleen or lymph nodes.

The kit is based on a cocktail of biotin-conjugated antibodies against CD8a, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, and Ter-119. The isolation of T cells is achieved by depletion of magnetically labeled cells.

Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.

The CD4+ T Cell Isolation Kit has been used for the following applications: For adoptive transfer experiments1,2 , T cell proliferation assays1,2 , Coculture with dendritic cells3 and Cytokine analysis3 experiments.

美天旎小鼠CD4+ T细胞分离试剂盒II研究背景：

在CD4 + T细胞分离试剂盒II已发展为隔离从小鼠脾细胞或淋巴结中悬浮的CD4 + T细胞的结细胞。非CD4+ T细胞，即细胞毒性T细胞，B细胞，NK细胞，树突细胞，巨噬细胞，粒细胞，内皮细胞和红系细胞通过间接呗磁性标记的使用生物素标记的抗体与抗生物素的混合物微珠。高纯度的CD4 + T细胞的隔离是通过实现耗尽磁标记的非目标细胞。

Miltenyi 美天旎小鼠CD4+ T细胞分离试剂盒II产品介绍：

Components

1 mL CD4+ T Cell Biotin-Antibody Cocktail,mouse: Cocktail of biotin-conjugated monoclonal antibodies against CD8a, CD11b,CD11c, CD19, CD45R (B220), CD49b (DX5),CD105, Anti-MHC class II, and Ter-119.

2 mL Anti-Biotin MicroBeads:

MicroBeads conjugated to monoclonal antibiotin antibodies (isotype: mouse IgG1).

Capacity

For 10*9 total cells, up to 100 separations.

Product format

All components are supplied in buffer containing stabilizer and 0.05% sodium azide.

Storage

Store protected from light at 2 − 8 °C. Do not freeze. The expiration date is indicated on the vial label.

Miltenyi 美天旎小鼠CD4+ T细胞分离试剂应用：

●CD4+ T细胞功能研究。

●研究CD4+ T细胞的活化信号的要求，诱导T细胞增殖，诱导T细胞无反应性等等

●研究CD4+ T细胞的信号转导。

●研究CD4+ T细胞细胞因子表达的调控。

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