产品英文名称：Miltenyi CD34 MicroBeads, human

产品中文名称：美天旎人CD34微珠试剂盒

Miltenyi CD34 MicroBead Kit 微珠试剂盒研究背景：

CD34抗原是一种单链跨膜糖蛋白表达于人类造血祖细胞，内皮祖细胞，血管内皮细胞，胚胎成纤维细胞，和一些细胞在胎儿和成人神经组织。在CD34微珠套件包直接结合微珠到CD34抗体对CD34表达细胞的磁性标记从外周血，脐带血，骨髓，血液分离收获，或分化的ES和iPS细胞。造血祖细胞，呈现在外周血中约0.05-0.2％的频率，在脐带血0.1-0.5％，并且在骨髓0.5-3％，可迅速而有效地丰富了。

美天旎人CD34微珠试剂盒产品介绍：

Components

2 mL CD34 MicroBeads, human:

MicroBeads conjugated to monoclonal mouseanti-human CD34 antibodies (isotype: mouseIgG1).

2 mL FcR Blocking Reagent, human:

Human IgG.

or

10 mL CD34 MicroBeads, human:

MicroBeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype: mouseIgG1).

10 mL FcR Blocking Reagent, human:

Human IgG.

Capacity

2 mL: For 2x10*9 total cells, up to 20 separations.

or

10 mL: For 10*10 total cells, up to 100 separations.

Product format

CD34 MicroBeads are supplied in buffer containing stabilizer and 0.05% sodium azide.

Storage

Store protected from light at 2?8 °C. Do not freeze. The expiration date is indicated on the vial label.

Miltenyi CD34 MicroBead Kit 微珠试剂盒应用：

●正选择或细胞表达人CD34抗原。

●分离造血祖细胞。

●分离内皮祖细胞（EPCs）的。

●从分化的ES和iPS细胞培养中分离CD34+祖细胞

●在体外分化的研究。

●研究恶性血液病。

美天旎人CD34微珠试剂盒产品英文描述：

The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:

peripheral blood (PBMCs)

bone marrow1

leukapheresis product

cord blood3

differentiated ES and iPS cell lines.

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.

Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Isolated cells are suitable for various downstream applications, e.g., flow cytometric analysis, expansion, transduction10 or differentiation5 in culture, reprogramming3, gene expression analyses, or transplantation into humanized mice.