产品英文名称：Miltenyi Stemgent Mouse Laminin

产品中文名称：美天旎Stemgent Pluriton小鼠层粘连蛋白

层粘连蛋白的作用：

层粘连蛋白是重组基底膜（RBM），分离出的小鼠Engelbreth-的Holm-群（EHS）肿瘤的主要成分。这种糖蛋白由包含促进细胞附着和其它生物活性位点三种多肽链（A，B，和g）。胚胎干（ES）细胞表达的受体层粘连蛋白结合在本机层粘连蛋白分子的这些位点。采用层粘连蛋白作为生长基质时，应考虑存在RBM凝胶肽生长因子可能混淆实验。以纯的形式，层粘连蛋白足以支持优异的ES细胞和iPS细胞生长中的未分化状态中所定义的媒体使用和不使用的小分子激酶抑制剂。此外，层粘连蛋白可以作为一种替代的小鼠胚胎成纤维细胞（MEF）饲养细胞用于传播多能干的人细胞系，有或无血清，并可以被用来支持推导和促进谱系限制性祖细胞的分化。

Miltenyi Stemgent Mouse Laminin 小鼠层粘连蛋白产品介绍：

Size

1 mg

Concentration

1 mg/ml

Formulation

0.05 M Tris-HCl, pH 7.4, and 0.15 M NaCl

Source

Mouse EHS tumor

Purity

Greater than 98% pure by SDS-PAGE with no detectable entactin or proteoglycan (Figure 1).

Storage and Stability

Store the vial of Mouse Laminin at -20°C to -80°C. Mouse Laminin is stable for 3 months from date of receipt when stored as directed.

Thaw the vial of Mouse Laminin slowly on ice or at 4°C. Avoid repeated freeze-thaws as it can result in loss of activity.

Quality Control

Mouse Laminin is free of bacteria, fungi, mycoplasma, and murine specific viruses. Endotoxin level is less than 5 EU/ml. Mouse Laminin supports axonal outgrowth of dorsal root ganglion (DRG) as well as the attachment of mouse and human ES cells (Figure 2).

美天旎Stemgent Pluriton小鼠层粘连蛋白产品英文描述：

Part of the extracellular matrix (ECM) family of glycoproteins, laminin is an integral part of the structural scaffolding in most tissues1-2. In its pure form, laminin alone has proven to be sufficient in supporting embryonic stem (ES) and induced pluripotent stem (iPS) cell growth in the undifferentiated state in defined media with and without small molecule kinase inhibitors3. Laminin was first isolated from the mouse Engelbreth-Holm-Swarm (EHS) tumor, and is the main component of the reconstituted basement membrane (RBM)4. In addition, laminin can serve as an alternative to RBM for the attachment of pluripotent human cell lines with or without serum, and can be used to support derivation and promote the differentiation of lineage-restricted progenitor cells.

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