产品英文名称:Covance RNA Polymerase II 8WG16 Monoclonal Antibody
产品中文名称:Covance RNA聚合酶II8WG16单克隆抗体
Covance RNA Polymerase RNA聚合酶II8WG16单克隆抗体英文描述:
Description | Monoclonal Antibody against RNA Polymerase II |
Also Known As | DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit |
Intended Use | ** **Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. ** |
Clone | 8WG16 |
Form | Ascites |
Host | Mouse |
Species Reactivity | Extensive (Yeast to Human) |
IsoType | IgG2a |
Specificity | The antibody 8WG16 was developed using RNA polymerase (RNA pol II) purified from wheat germ extract. It recognizes the C-terminal heptapeptide repeat present on the largest subunit of pol II. Clone 8WG16 has been used to investigate the role of the C-terminal domain of the 220kD RNA pol II subunit in the formation of the transcription complex. |
Covance RNA Polymerase RNA聚合酶II8WG16单克隆抗体应用:
免疫荧光(IF),免疫印迹法(WB),免疫沉淀(IP),聚合酶II的纯化和功能研究。
Covance RNA Polymerase RNA聚合酶II8WG16单克隆抗体使用注意事项:
建议工作稀释度:最佳工作稀释度应为每个特定的实验条件来确定。
WB:1:500
IF:1:50 - 1:100
IP:1:100
Motes:预测分子量=220 kDa的
储存:储存于-20°C。经初步解冻,分配到分装并储存于-20°C。但应避免反复冻融,以防止变性的抗体。对于长期储存,保持抗体在-80°C。
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