英文名称：Recombinant Mouse Interleukin-6

中文简称：【mIL 6】重组小鼠白介素-6

Recombinant Mouse Interleukin-6 【mIL 6】重组小鼠白介素-6 细胞因子产品描述：

Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense，acute phase reactions，immune responses and hematopoiesis.

小鼠白介素-6重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有187个氨基酸，分子量为21709Da。该小鼠白介素-6重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Mouse Interleukin-6 【mIL 6】重组小鼠白介素-6 细胞因子产品使用须知：

溶解：提供的小鼠白介素-6(mIL 6)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含小鼠白介素-6(mIL 6)的溶液（不含添加剂）中冻干产生的。我们建议加10mM HCl以配制工作储存液到0.1-1.0 mg/ml，后继实验过程中可根据实验要求进一步稀释。

保存：小鼠白介素-6(mIL 6)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Mouse Interleukin-6 【mIL 6】重组小鼠白介素-6 细胞因子产品英文描述：

Synonyms

IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.

Introduction

Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.

Description

Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.

The IL-6 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance （物理特征）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Mouse Il-6 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 96.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

FPTSQVRRGD FTEDTTPNRP VYTTSQVGGL ITHVLWEIVE MRKELCNGNS DCMNNDDALA ENNLKLPEIQ RNDGCYQTGY NQEICLLKIS SGLLEYHSYL EYMKNNLKDN KKDKARVLQR DTETLIHIFN QEVKDLHKIV LPTPISNALL TDKLESQKEW LRTKTIQFIL KSLEEFLKVT LRSTRQT.

Biological Activity （生物活性）

The ED50 as determined by the dose-dependant stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is < 0.02 ng/ml, corresponding to a specific activity of > 50,000,000 units/mg.