英文名称：Recombinant Human Granulocyte-Colony Stimulating Factor，CHO

中文简称：【G CSF CHO】重组人粒细胞集落刺激因子,CHO表达

Recombinant Human Granulocyte-Colony Stimulating Factor，CHO 【G CSF CHO】重组人粒细胞集落刺激因子,CHO表达 细胞因子产品描述：

粒细胞集落刺激因子(granulocyte colony stimulating factor,G-CSF)基因全长2.5kb，包括5个外显子和4个内含子，人G-CSF基因位于17号染色体，与小鼠G-CSF基因约有73％同源性,与IL-6无论在基因水平以及氨基酸水平上都有很高同源性，包括外显子、内含子组成，Cys数目，两对二硫键位置以及分子的三级结构等。G-CSF主要作用于中性粒细胞系造血细胞的增殖、 分化和活化。在体外G-CSF刺激骨髓造血祖细胞中中性粒细胞集落的形成，延长成熟中性粒细胞的存活时间，活化中性粒细胞如促进ADCC，超氧阴离子的产生和碱性磷酸酶的合成。G-CSF还具有对人粒细胞、单核细胞、成纤维细胞、平滑肌细胞以及成肌纤维细胞的趋化作用。肿瘤患者注射G-CSF后可提高血循环中中性粒细胞的水平，这种作用可能与缩短某些骨髓细胞进入S期的时间以及增加生成粒细胞的祖细胞数量有关。

人粒细胞集落刺激因子，CHO表达重组蛋白是在CHO中国仓鼠卵巢细胞中重组表达的，是一条单链、糖基化的多肽链，包含有174个氨基酸，分子量为18kDa。该人粒细胞集落刺激因子，CHO表达重组蛋白最终由标准的色谱技术纯化出。

来源宿主---CHO中国仓鼠卵巢细胞中重组表达

Recombinant Human Granulocyte-Colony Stimulating Factor，CHO 【G CSF CHO】重组人粒细胞集落刺激因子,CHO表达 细胞因子使用须知：

溶解：提供的人粒细胞集落刺激因子,CHO表达(G CSF CHO)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人粒细胞集落刺激因子,CHO表达(G CSF CHO)的10mM盐酸（pH 6.5），0.4mg Tween 20，100mg甘露醇，160 mg左型精氨酸，40 mg苯基丙氨酸和4mg甲硫氨酸中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人粒细胞集落刺激因子,CHO表达(G CSF CHO)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Granulocyte-Colony Stimulating Factor，CHO 【G CSF CHO】重组人粒细胞集落刺激因子,CHO表达 细胞因子英文描述：

Synonyms

SF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.

Introduction

Granulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. GCSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils.

Description

Granulocyte Colony Stimulating Factor Human Recombinant produced in CHO cells is a single, glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18 kDa.

G-CSF is purified by proprietary chromatographic techniques.

Source （来源）

Chinese Hamster Ovary Cells (CHO).

Physical Appearance （物理特征）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

G-CSF was lyophilized from a concentrated (1mg/ml) Phosphate- Buffered Saline, pH 7.4.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution G-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Amino acid sequence （氨基酸序列）

TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.

Purity （纯度）

Greater than 97.0% as determined by:

a) Analysis by RP-HPLC.

b) Analysis by SDS-PAGE.

Biological Activity （生物活性）

The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg.

Protein content （蛋白质含量）

GCSF quantitation was carried out by two independent methods:

1) UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2) Analysis by RP-HPLC, using a calibrated solution of Granulocyte Colony Stimulating Factor as a Reference Standard.