英文名称：Recombinant Mouse Epidermal Growth Factor

中文简称：【mEGF】重组小鼠表皮生长因子

Recombinant Mouse Epidermal Growth Factor 【mEGF】重组小鼠表皮生长因子 细胞因子产品描述：

EGF（Epidermal Growth Factor）中文名称表皮生长因子，是一类重要的生长因子。EGF是有53个氨基酸分子组成的肽链，链中含有6个半胱氨酸分子，半胱氨酸分子间形成稳定的双硫键，使整条肽链成为三个环联部分的活性物质。EGF的主要作用是促进皮肤细胞的增殖、分化，加速受伤表皮细胞的修复，加速新陈代谢，提高新细胞在皮肤中所占的比例，它能起到延缓皮肤细胞衰老，使皮肤滋润光滑的作用。还有抑制胃酸分泌等特殊的效果，在医药上有很广的用途。

小鼠表皮生长因子重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有53个氨基酸（包括3个分子间二硫键），分子量为6kDa。该小鼠表皮生长因子重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Mouse Epidermal Growth Factor 【mEGF】重组小鼠表皮生长因子 细胞因子产品使用须知：

溶解：提供的小鼠表皮生长因子(mEGF)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml小鼠表皮生长因子(mEGF)的1x PBS （pH 7.4）溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：小鼠表皮生长因子(mEGF)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Mouse Epidermal Growth Factor 【mEGF】重组小鼠表皮生长因子 细胞因子产品英文描述：

Synonyms

Urogastrone, URG, EGF.

Introduction

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description

Epidermal Growth Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.

The EGF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing 1x PBS pH-7.4.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 97.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Tyr-Pro-Gly, which agrees with the sequence of native Epidermal Growth Factor human.

Biological Activity （生物活性）

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10,000,000 units/mg.

Protein content （蛋白质含量）

Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 3 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.