英文名称：Recombinant Human Insulin

中文简称：【Insulin】重组人胰岛素

Recombinant Human Insulin 【Insulin】重组人胰岛素 细胞因子产品描述：

胰岛素降低血糖浓度。它增加细胞渗透性单糖、氨基酸和脂肪酸。戊糖磷酸循环,加速糖酵解和糖原合成在肝脏。

人胰岛素重组蛋白是在大肠杆菌中重组表达的，是由两条链非糖基化多肽链组成的二聚体；每条多肽链包含有51个氨基酸，分子量为5807Da。该人胰岛素重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Human Insulin 【Insulin】重组人胰岛素 细胞因子产品使用须知：

溶解：提供的人胰岛素(Insulin)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人胰岛素(Insulin)的溶液（不含添加剂）中冻干产生的。我们建议加无菌的0.005N HCl以配制工作储存液到1 mg/ml。

保存：人胰岛素(Insulin)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Insulin 【Insulin】重组人胰岛素 细胞因子产品英文描述：

Introduction

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Description

Insulin Human Recombinant produced in E.Coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Insulin is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance（外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

Solubility（溶解性）

It is recommended to reconstitute the lyophilized Insulin in sterile 0.005N HCl not more than 1 mg/ml.

Stability （稳定性）

Lyophilized Insulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity（纯化）

Greater than 98.0% as determined by

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Biological Activity （生物活性）

The Biological Activity was determined to be 28 units/mg.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.