英文名称：Recombinant Human Fibroblast Growth Factor-acidic

中文简称：【FGF 1】重组人酸性成纤维细胞生长因子

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重组人酸性成纤维细胞生长因子产品描述：

酸性成纤维细胞生长因子(acidic fibroblast growth factor，aFGF) 是成纤维细胞生长因子家族成员之一,对中胚层及神经外胚层来源的细胞具有促分裂的作用。aFGF主要分布于大脑,它以自分泌或旁分泌的方式作用于周围细胞,但其本身缺乏信号肽结构。aFGF蛋白由154个氨基酸残基组成，等电点5～7，通过与其受体结合启动信号转导机制从而诱导多种细胞分化、增殖。

人酸性成纤维细胞生长因子重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有141个氨基酸，分子量为15967Da。该人酸性成纤维细胞生长因子重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重组人酸性成纤维细胞生长因子产品使用须知：

溶解：提供的人酸性成纤维细胞生长因子(FGF 1)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人酸性成纤维细胞生长因子(FGF 1)的10mM 磷酸钠（pH 7.5)和75mM NaCl溶液中过滤灭菌并冻干产生的。我们建议加无菌的4℃ 18MΩ-cm水以配制工作储存液到0.1-0.25mg/ml。将样本在4℃中竖直放置5分钟后，离心去除沉淀物。

保存：人酸性成纤维细胞生长因子(FGF 1)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装后置于-18℃冻存。请避免反复冻融。

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重组人酸性成纤维细胞生长因子产品英文描述：

Synonyms

HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.

Introduction

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.

Three alternatively spliced variants encoding different isoforms have been described.

The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Description

Fibroblast Growth Factor-acidic Human Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of approximately 15.8kDa.

The FGF acidic is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing PBS, pH 7.4.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ-cm H2O at 4 degrees Celsius at a concentration of 0.1mg-0.25mg per 1ml. Allow sample to sit for 5 min. at 4 degrees, spin to remove precipitant.

Stability （稳定性）

Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-a should be stored at 4°C between 2-7 days and for future use below -18°C.

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Biological Activity （生物活性）

The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is <0.5 ng/ml, corresponding to a specific activity of > 2.0 x 1,000,000IU/mg.