英文名称：Recombinant Human Hepatocyte Growth Factor，CHO

中文简称：【HGF CHO】重组人肝细胞生长因子,CHO表达

Recombinant Human Hepatocyte Growth Factor，CHO 【HGF CHO】重组人肝细胞生长因子,CHO表达 细胞因子产品描述：

肝细胞生长因子（hepatocyte growth factor，HGF）最初作为一种肝细胞有丝分裂原是从肝部分切除大鼠的血清中分离得到的。HGF是来源于间充质的多效生长因子，可以促进多种细胞的分裂和生长。以旁分泌、内分泌、自分泌形式在保护器官，延长组织老化方面发挥作用。HGF首先以无活性的单链蛋白（HGF SF）形式分泌，经肝细胞生长因子激活因子切割成成熟的异二聚体HGF，机体内HGFA可激活HGF SF前体，使之从单链前体变成双链活性形式，是调节细胞外HGF SF活性的关键步骤。HGF在促进肝细胞再生的同时参与正常组织的损伤后修复、细胞的运动以及肿瘤的形成、浸润和转移、分化以及肿瘤血管行程中发挥重要的作用。

人肝细胞生长因子，CHO表达重组蛋白是在CHO中国仓鼠卵巢细胞中重组表达的，是由两条非糖基化多肽链，即463个氨基酸的α链和234个氨基酸的β链组成的异源二聚体，总分子量为75kDa。该人肝细胞生长因子，CHO表达重组蛋白最终由标准的色谱技术纯化出。

来源宿主---CHO中国仓鼠卵巢细胞中重组表达

Recombinant Human Hepatocyte Growth Factor，CHO 【HGF CHO】重组人肝细胞生长因子,CHO表达 细胞因子产品使用须知：

溶解：提供的人肝细胞生长因子,CHO表达(HGF CHO)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人肝细胞生长因子,CHO表达(HGF CHO)的0.02% Tween 80，磷酸盐缓冲液（pH 7.4）中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人肝细胞生长因子,CHO表达(HGF CHO)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Hepatocyte Growth Factor，CHO 【HGF CHO】重组人肝细胞生长因子,CHO表达 细胞因子产品英文描述：

Synonyms

Scatter Factor (SF), Hepatopoietin (HPTA), HGF.

Introduction

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3and GM-CSFto stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.

Description

Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 75kDa.

The HGF is purified by proprietary chromatographic techniques.

Source （来源）

Chinese Hamster Ovary Cells.

Physical Appearance （外表）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability（稳定性）

Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 97.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

Agrees with the sequence of native human HGF.

Biological Activity （生物活性）

The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40ng/ml corresponding to a Specific Activity of 25,000-50,000IU/mg.

Protein content （蛋白质含量）

Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.