英文名称：Recombinant Human Keratinocye Growth Factor

中文简称：【KGF】重组人角质细胞生长因子

Recombinant Human Keratinocye Growth Factor 【KGF】重组人角质细胞生长因子 描述：

人角质细胞生长因子重组蛋白为纤维母细胞生长因子(FGF)的家庭。FGF家庭成员拥有广泛的促有丝分裂细胞生存活动,并参与多种生物过程,包括胚胎发育、细胞生长、形态发生、组织修复、肿瘤生长和入侵。FGF7是一个强有力的上皮特异性生长因子的促有丝分裂的活动主要是表现在角质细胞而不是在成纤维细胞和内皮细胞。研究该基因的老鼠和老鼠同系物涉及角色在上皮的形态发生,reepithelialization伤口,头发和早期肺器官发生发展。

人角质细胞生长因子重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有164个氨基酸，分子量为18995Da。该人角质细胞生长因子重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Human Keratinocye Growth Factor 【KGF】重组人角质细胞生长因子实验须知：

溶解：提供的人角质细胞生长因子(KGF)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人角质细胞生长因子(KGF)的溶液（不含添加剂）中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人角质细胞生长因子(KGF)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Keratinocye Growth Factor 【KGF】重组人角质细胞生长因子 英文描述：

Synonyms

HBGF-7, FGF7, FGF-7, KGF.

Introduction

KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Description

Keratinocyte Growth Factor-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton.

The FGF-7 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2?m filtered solution in 20mM PB, pH 8.0, 1M NaCl.

Solubility

It is recommended to reconstitute the lyophilized Keratinocyte Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability (稳定性）

Lyophilized Keratinocyte Growth Factor1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF7 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 96.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT.

Biological Activity (生物活性）

The biological activity was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 <10ng/ml, corresponding to a Specific Activity of 1.0×105 IU/mg.

Protein content

Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard.