英文名称：Recombinant Human Insulin Like Growth Factor-1

中文简称：【IGF1】重组人胰岛素样生长因子-1

Recombinant Human Insulin Like Growth Factor-1 【IGF1】重组人胰岛素样生长因子-1 细胞因子产品描述：

胰岛索样生长因子一1(Insulin―like Growth Factor―l，IGF 1)是胰岛素样生长因子的重要成员，与胰岛索高度同源。这个系统的其它成员还包括IGF Ⅱ、1GF―l受体(IGFIR)、IGF Ⅱ受体(IGFIIR)以及4种胰岛素生长因子结合蛋白(IGF BP-1、-2、-3、-4)。其中胰岛素样生长因子-1（Insulin-like growth factor-1，IGF-1）由70个氨基酸组成的单链碱性多肽分子，分子量为7649。它来源于体内许多细胞，通过自分泌、旁分泌和内分泌机制并与IGF-1受体（IGF-1R）结合发挥多种生理作用。作为一类促进细胞生长、具有胰岛素样代谢效应的因子。IGF-1受营养状态、激素、遗传等多因素的调节，在心血管疾病、内分泌代谢病及肿瘤等的病理生理过程中发挥重要作用。

人胰岛素样生长因子-1重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有70个氨基酸，分子量为7655Da。该人胰岛素样生长因子-1重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达。

Recombinant Human Insulin Like Growth Factor-1 【IGF1】重组人胰岛素样生长因子-1细胞因子产品实验须知：

溶解：提供的人胰岛素样生长因子-1(IGF1)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含人胰岛素样生长因子-1(IGF1)的溶液（不含添加剂）中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人胰岛素样生长因子-1(IGF1)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Insulin Like Growth Factor-1 【IGF1】重组人胰岛素样生长因子-1 细胞因子产品英文描述：

Synonyms

Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.

Introduction

The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).

Description

Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa.

IGF-I is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized without any additives.

Solubility

It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC

(b) Analysis by SDS-PAGE.

Amino acid sequence

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

Biological Activity

The activity as determined by the dose-dependent proliferation of mouse FDC-P1 is less than 1.0 ng/ml, corresponding to a specific activity of 1x106 units/mg.

Protein content

Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.