产品英文名称：StemRD DKK-500 DKK-1,Human,500ug

产品中文名称：StemRD DKK-500 人源Dkk-1蛋白WNT信号通路抑制剂,500ug

StemRD DKK-100 DKK-1,Human,500ug 产品简介

DKK-1是重组人Dickkopf相关蛋白1的简称，是DKK蛋白家族（DKK1-4）的一员。DKK最初被认为是非洲爪蟾蜍的前期成形细胞。通过机理 研究发现DKK-1通过与共同受体LRP5/6一起形成抑制复合物，可以有效抑制Wnt/β-catenin信号通路。研究人员发现敲除DKK-1基因小 鼠的脑部无法形成，从而证实Wnt/β-catenin信号通路的抑制在脊椎动物的后模式与前模式发展中必不可少。

StemRD公司生产的人重组StemRD DKK-500 DKK-1,Human,500ug 蛋白干细胞生长分化调整因子来源于人的293细胞，是含有235个氨基酸片段，分子量为3740kDa的糖蛋白。通过诸如HPLC等一系列色谱分析方法对其进行纯化。

Wnt 信号转导通路参与了多种生物学过程的调控, 包括胚胎的生长和形态发育、组织的稳定、能量代谢的平衡以及干细胞的维持。Wnt 通路的失调与人类重大疾病有密切联系。

近年来人们在对干细胞的研究中发现,Wnt 信号通路对于表皮干细胞、肠干细胞、造血干细胞、神经干细胞、胚胎干细胞以及肿瘤干细胞的维持都起着重要的调控作用。所以, 深入研究Wnt 信号通路的调控机理, 寻找药物治疗的靶蛋白,是近年来生物学和医学研究的热门领域。

StemRD DKK-500 人源Dkk-1蛋白WNT信号通路抑制剂,500ug 产品特性

» StemRD DKK-500 人源Dkk-1蛋白干细胞生长分化调整因子WNT信号通路抑制剂,500ug鉴定纯度大于90%

» StemRD DKK-500 人源Dkk-1蛋白干细胞生长分化调整因子WNT信号通路抑制剂,500ug生物活性在培养人细胞中用TCF受体基因检测确定，拮抗剂WNT-3a 100 ng/mL时其IC50在100 - 500 ng/ml之间

StemRD DKK-500 DKK-1,Human,500ug 保存方法

The lyophilized protein is stable for at least one year if stored at -80 degreeC. Reconstituted protein is stable for at least four weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid

repeated freeze and thaw.

StemRD DKK-500 DKK-1,Human,500ug 产品英文描述

DKK-1, Dickkopf-related protein-1, is a member of the DKK protein family which includes DKK-1 through DDK-4. DKK was originally identified as a head-forming molecule in Xenopus. Mechanistic studies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway by forming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling is essential for posterior patterning and anterior development in vertebrates, supported by the discovery that DKK-1 knockout mice lack head formation.

StemRD expressed recombinant human Dkk-1,human DKK-500,500ug in human 293 cells as a 37-40 kDa glycoprotein containing 235 amino-acid residues. It is purified by aseries of chromatography including HPLC.

•Dkk-1,human DKK-500,500ug Purity: Greater than 90% as determined by SDS-PAGE analysis

•Dkk-1,human DKK-500,500ug Biological Activity: The activity was determined by using a TCF reporter gene assay in cultured human cells. The IC50 ranges from 100 - 500 ng/ml in the inhibition of 100 ng/mL WNT-3a activity. Activity in other assays should be determined by each individual setting.

StemRD 人源Dkk-1蛋白WNT信号通路抑制剂系列产品订购信息

StemRD DKK-025 25ug 优惠价格请来电咨询

StemRD DKK-100 100ug 优惠价格请来电咨询

StemRD DKK-500 500ug 优惠价格请来电咨询

StemRD DKKFC-050 50ug 优惠价格请来电咨询

StemRD DKKFC-250 250ug 优惠价格请来电咨询

StemRD DKKFC-1000 1000ug 优惠价格请来电咨询

For research use only. Not intended for human or animal diagnostic or therapeutic uses.