货号：PA121041

品牌：Pierce

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio GLUT1 Polyclonal Antibody for Western Blot, IF, ICC and IHC (P)

GLUT1 Antibody (PA1-21041)

Immunohistochemical analysis of Glucose Transporter 1 in formalin-fixed, paraffin-embedded human esophagous using a Glucose Transporter 1 polyclonal antibody (Product # PA1-21041).

Product Details References Bioinformatics Documents

Details
Tested species reactivity	Human, Rat
Published species reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide to the C-terminal region of human GLUT1. The peptide sequence is considered commercially sensitive.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/ml
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.09% sodium azide
Storage Conditions	4° C, do not freeze

Tested Applications	Dilution *
Immunocytochemistry (ICC)	Assay-dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (Paraffin) (IHC (P))	1:200
Western Blot (WB)	0.5 µg/ml for 2 hrs

* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

Published Applications
Western Blot (WB)	See 1 publications below
Immunohistochemistry (IHC)	See 3 publications below

Product Specific Information

PA1-21041 detects glucose transporter (GLUT1) in human and rat samples. PA1-21041 has been successfully used in immunofluorescence, immunohistochemistry (paraffin) and Western blot procedures. Staining of formalin fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 minutes. PA1-21041 immunogen corresponds to a synthetic peptide to the C-terminal region of human GLUT1. The peptide sequence is considered commercially sensitive.

Background/Target Information

Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins designated as Glut 1 to 7 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%) all in the molecular weight range of 40 to 60 kDa. Individual members of the Glut family have predicted secondary structure characteristics of 12 membrane spanning domains of the transport carriers. Majority of the difference in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C-terminus and are present on either cell surface or intracellular sites. Some transporters exhibit dynamic trafficing between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are assymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood tetis barriers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References

WB (1)

• IHC (3)
  Western Blot

  Species	Dilution	Title	Authors	Journal (Vol:Pg)	Year
  Human	1:200	Immunolocalization of platelet-derived growth factor receptor-β (PDGFR-β) and pericytes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). PA1-21041 was used in western blot to determine the pericyte distribution in relation to N3ECD deposits in cerebral microvessels in patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy	Craggs LJ,Fenwick R,Oakley AE,Ihara M,Kalaria RN	Neuropathology and applied neurobiology (41:557)	2015

  Bioinformatics Protein Aliases:

  choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); erythrocyte/brain; glucose transporter type 1, erythrocyte/brain; GLUT-1; hepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1

  Gene Aliases:

  CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1

  UniProt ID:

  (Human) P11166, (Rat) P11167

  Entrez Gene ID:

  (Human) 6513, (Rat) 24778

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