产品英文名称:EasySep™ Human Whole Blood CD34 Positive Selection Kit
产品中文名称:EasySep™ 人血CD34阳性选择试剂盒
EasySep™ Human Whole Blood CD34 Positive Selection Kit 产品英文描述
The EasySep™ Human Whole Blood CD34 Positive Selection Kit is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells. If isolating CD34+ cells from fresh cord blod, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit (Catalog #18096). If isolating CD34+ cells from any other sample type, we recommend using the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056).
EasySep™ 人血CD34阳性选择试剂盒产品组分
• EasySep™ Human Whole Blood CD34 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep™ Magnetic Particles, 3 x 1.0 mL
• EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
EasySep™ Human Whole Blood CD34 Positive Selection Kit 推荐使用
The isolation of human CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection
EasySep™ 人血CD34阳性选择试剂盒保存方法
EasySep™ Human Whole Blood CD34 Positive Selection Cocktail
Stable at 4°C for 2 years. Do not freeze this product. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep™ Magnetic Nanoparticles EasySep
Stable at 4°C for 2 years. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep™ RBC Lysis Buffer 10X Concentrate EasySep
10X concentrate is stable at room temperature for 2 years. 1X lysis buffer is stable at 4°C for 3 months. Do not freeze.
EasySep™ Human Whole Blood CD34 Positive Selection Kit 实验资料
FACS Profile Results with EasySep® Human Buffy Coat/Whole Blood CD34 Positive Selection Kit
Starting with buffy coat, the CD34+ cell content of the enriched fraction typically ranges from 51.7% - 90.3% (reported as a percentage of viable CD45+ cells).
Typical EasySep™ Procedure for Human Cells
EasySep™ 人血CD34阳性选择试剂盒相关产品订购信息
Stemcell 10513 CD34 Antibody, Clone 8G12, PE-Conjugated Mouse Monoclonal Antibody to Human CD34 - PE Conjugated
Stemcell 10413 CD34 Antibody, Clone 8G12, FITC-Conjugated Mouse Monoclonal Antibody to Human CD34 - FITC Conjugated
Stemcell 10613 CD34 Antibody, Clone 8G12, APC-Conjugated Mouse Monoclonal Antibody to Human CD34 - PE Conjugated
Stemcell 07801 Lymphoprep™ Density Gradient Medium for the Isolation of Mononuclear Cells
Stemcell 20110 EasySep™ Red Blood Cell Lysis Buffer Red Blood Cell Lysis Buffer
Stemcell 07800 Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells
Stemcell 18001 The Big Easy EasySep™ Magnet Immunomagnetic Column-Free Magnet
Stemcell 20000 RoboSep™ RoboSep™ - The Fully Automated Cell Separator
Stemcell 18000 EasySep™ Magnet Immunomagnetic Column-Free Magnet
Stemcell 04034 MethoCult™ H4034 Optimum Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Human Cells
Stemcell 04435 MethoCult™ H4435 Enriched Methylcellulose Medium with Recombinant Cytokines and EPO for Human Cells
Stemcell 04434 MethoCult™ H4434 Classic Methylcellulose-Based Medium with Recombinant Cytokines for Human Cells
Stemcell 60013 Anti-Human CD34 Antibody, Clone 581 Mouse Monoclonal Antibody Against Human CD34
For research use only. Not intended for human or animal diagnostic or therapeutic uses.
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