产品英文名称: EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion

产品中文名称: EasySep™ 没有CD16耗尽的人单核细胞富集试剂盒

EasySep™ 没有CD16耗尽的人单核细胞富集试剂盒产品英文描述

The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD19, CD20, CD56, CD66b, CD123, glycophorin A and dextran-coated magnetic particles. The cocktail also contains and antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (Catalog #19059), which contains anti-CD16.

EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Recommended for

The isolation of human monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection

EasySep™ 没有CD16耗尽的人单核细胞富集试剂盒产品组成

• EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1.0 mL

• EasySep™ Magnetic Particles, 1.0 mL

EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion 保存方法

EASYSEP™ HUMAN MONOCYTE ENRICHMENT COCKTAIL WITHOUT CD16

DEPLETION

EASYSEP™ D MAGNETIC PARTICLES FOR MONOCYTES

Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.

EasySep™ 没有CD16耗尽的人单核细胞富集试剂盒实验资料

FACS Histogram Results Using EasySep® Human Monocyte Enrichment Kit Without CD16 Depletion

Starting with freshly prepared peripheral blood mononuclear cells, the CD14+ cell content of the enriched fraction typically ranges from 73% - 81%. Slightly lower CD14+ cell purities may be obtained from samples that contain a large number of CD16+ cells.

Typical EasySep™ Procedure for Human Cells

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For research use only. Not intended for human or animal diagnostic or therapeutic uses.