产品英文名称: EasySep™ RosetteSep™ Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells

产品中文名称: EasySep™ RosetteSep™ CD4+ CD127lowCD25+调节性T细胞完整试剂盒

EasySep™ RosetteSep™ Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells 产品英文描述

The Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells is a simple two-step method that is designed to isolate CD4+CD127lowCD49d-CD25+ cells from whole blood or buffy coat. CD4+CD127lowCD49d- T cells are enriched from whole blood or buffy coat by negative selection using RosetteSep™ CD4+CD127lowCD49d- T Cell Pre-Enrichment Cocktail. The RosetteSep™ cocktail contains a combination of antibodies that is directed against non-CD4+CD127lowCD49d- T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Ficoll-Paque™ PLUS (Catalog #07957), the unwanted cells pellet along with the RBCs. The purified cells are present as an enriched population at the interface between the plasma and the buoyant density medium. The enriched cells are then selected for CD25+ cells by positive selection using EasySep™ Human CD25 Positive Selection Kit. Cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

EasySep™ RosetteSep™ Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells 推荐使用

The isolation of human CD4+CD127lowCD49d-CD25high regulatory T cells from whole blood or buffy coat by positive selection

EasySep™ RosetteSep™ Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells 产品组成

• RosetteSep™ Human CD4+CD127lowCD49d- Pre-Enrichment Cocktail, 5 x 2 mL

• EasySep™ Human CD25 Positive Selection Cocktail, 1 mL

• EasySep™ Magnetic Particles, 1 mL

EasySep™ RosetteSep™ Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells 保存方法

ROSETTESEP® HUMAN CD4+CD127lowCD49d

REGULATORY T CELL PRE-ENRICHMENT COCKTAIL

Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not use cocktail if vial contents have leaked. Unused cocktail may be disposed of according to standard laboratory procedures for non-hazardous liquids. Do not freeze this product. This product may be shipped at room temperature (15 – 25°C), and should berefrigerated upon receipt.

EasySep™ RosetteSep™ Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells 订购信息

Stemcell 18000 EasySep™ Magnet Immunomagnetic Column-Free Magnet

Stemcell 18001 The Big Easy EasySep™ Magnet Immunomagnetic Column-Free Magnet

Stemcell 20000 RoboSep™ RoboSep™ - The Fully Automated Cell Separator

For research use only. Not intended for human or animal diagnostic or therapeutic uses.