货号：APT242

品牌：Merck Millipore

规格：100assays

目录价：￥6959.00

市场价格：￥5915.15

会员价格：￥5567.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: MitoLight® Mitochondrial Apoptosis Detection Kit View All» Trade Name:

• MitoLight

• Chemicon (Millipore)
  View All» Qty/Pk: 100 assays View All» Product Overview: Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. MitoLight™ partitions differently in healthy cells than in apoptotic cells. Therefore, it has been possible to use a fluorescence ratioing technique to study mitochondrial membrane potentials. In healthy cells, the dye accumulates and aggregates in the mitochondria, giving off a bright red fluorescence (λem = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing green (λem = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530) and PI channel for red aggregates (Ex/Em = 488/585). View All» Key Applications: Flow Cytometry View All» Species Reactivity: Mammals View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Components:
  • MitoLight™ (Part No. 71616): 100 μL in DMSO.

  • 10X Incubation Buffer (Part No. 71614): 20 mL.
    View All» Brand Family: Chemicon View All» Storage Conditions: Store kit materials at -20°C up to their expiration date. After thawing, unused kit components can be refrozen. For best results, aliquot the MitoLight™ reagent into separate vials to avoid repeated freeze/thaw cycles. PROTECT REAGENTS FROM LIGHT. View All» Detection Methods: Fluorescent View All» Product Name: MitoLight® Mitochondrial Apoptosis Detection Kit View All» Materials Required but Not Delivered: · Microcentrifuge and 1.5 mL Microcentrifuge tubes · 37°C CO2 incubator · Flow Cytometer · Glass slides with glass coverslips View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://16ao.com/thread-161013.htm
    © Merck Millipore代理APT242 MitoLight Mitochondrial Apoptosis Detection Kit;store at -20℃，产品报价联系微信jinshanbio