货号：APT166

品牌：Merck Millipore

规格：EA

目录价：￥5604.00

市场价格：￥4763.40

会员价格：￥4483.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Caspase 5 Fluorometric Activity Assay Kit, WEHD View All» Trade Name: Chemicon (Millipore) View All» Qty/Pk: 100 assays View All» Product Overview: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-5 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. The assay is based on detection of cleavage of substrate WEHD-AFC (7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (lmax=400 nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (lmax=505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-5 activity. For Research Use Only; not for use in diagnostic procedures View All» Key Applications: Activity Assay View All» Species Reactivity: Mammals View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. View All» UniProt Summary: FUNCTION: SwissProt: P51878 # Mediator of programmed cell death (apoptosis).SIZE: 418 amino acids; 47814 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits.TISSUE SPECIFICITY: Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.SIMILARITY: SwissProt: P51878 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. View All» Components:

• Cell Lysis Buffer - 100 mL

• 2X Reaction Buffer - 4 x 2 mL.

• DTT (1 M): 400 μL

• WEHD-AFC Substrate (1 mM): 500 μL
  View All» Brand Family: Chemicon View All» UniProt Number: P51878 View All» Storage Conditions: We recommend storing reagents at -20°C upon arrival. (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening) upon arrival. The performance of this product is guaranteed for 6 months. View All» Detection Methods: Fluorescent View All» Gene Symbol:
  • CASP5

  • ICH3

  • ICEREL-III

  • CASP-5

  • ICE

  • ICE(rel)III

  • relIII)

  • ICErel-III

  • MGC141966

  • ICH-3

  • EC 3.4.22.58 [Contains: Caspase-5 subunit p20

  • Caspase-5 subunit p10].
    View All» Product Name: Caspase 5 Fluorometric Activity Assay Kit, WEHD View All» Entrez Gene Number: NM_004347.1 View All» Materials Required but Not Delivered: · Microcentrifuge and 1.5 mL Microcentrifuge tubes · 37°C water bath or incubator · Fluorometer equipped with a 400-nm excitation filter and 505-nm emission filter. · 96 well plate View All»
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