货号：APT161

品牌：Merck Millipore

规格：EA

目录价：￥6189.00

市场价格：￥5260.65

会员价格：￥4951.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Caspase 1 Colorimetric Assay Kit, YVAD View All» Trade Name: Chemicon (Millipore) View All» Qty/Pk: 100 assays View All» Product Overview: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's ICE/Caspase-1 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-1 activity. For research use only; Not for use in diagnostic procedures View All» Key Applications: Activity Assay View All» Species Reactivity: Mammals View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. View All» UniProt Summary: FUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.SIZE: 404 amino acids; 45159 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. View All» Components:

• Cell Lysis Buffer - 100 mL

• 2X Reaction Buffer - 4 x 2 mL.

• DTT (1 M): 400 μL

• YVAD-pNA Substrate: 500 μL

• Dilution Buffer: 100 mL
  View All» Brand Family: Chemicon View All» UniProt Number: P29466 View All» Storage Conditions: We recommend storing reagents at -20°C upon arrival. (Store Cell Lysis Buffer and 2X Reaction Buffer and dilution buffer at 4°C after opening). The performance of this product is guaranteed for 6 months. View All» Detection Methods: Chromogenic View All» Gene Symbol:
  • CASP1

  • IL1BC

  • p45

  • ICE

  • IL1BCE

  • P45

  • IL-1BC

  • IL1B-convertase

  • CASP-1

  • EC 3.4.22.36 [Contains: Caspase-1 subunit p20

  • Caspase-1 subunit p10].
    View All» Product Name: Caspase 1 Colorimetric Assay Kit, YVAD View All» Entrez Gene Number:
    • NM_001223.3

    • NM_033292.2

    • NM_033293.2

    • NM_033294.2

    • NM_033295.2
      View All» Alternate Names: ICE View All» Materials Required but Not Delivered: · Microcentrifuge and 1.5 mL Microcentrifuge tubes · 37°C water bath or incubator · Spectrophotometer · 96 well plate View All»
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