货号：APT110

品牌：Merck Millipore

规格：

目录价：￥8548.00

市场价格：￥7265.80

会员价格：￥6838.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Apo-Direct TUNEL Assay Kit View All» Trade Name:

• ApoDIRECT

• Chemicon (Millipore)
  View All» Qty/Pk: 50 assays View All» Product Overview: The CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA. View All» Key Applications: Flow Cytometry View All» Species Reactivity: Vertebrates View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Components:
  • The following components are included with the APO-DIRECT™ Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.

  • Positive Control Cells,Brown cap

  • Negative Control Cells, Natural cap

  • Wash Buffer, Blue cap

  • Reaction Buffer, Green cap

  • TdT Enzyme, Yellow cap

  • F-dUTP, Orange cap

  • Rinsing Buffer, Red cap

  • PI/RNase Staining Buffer, Amber bottle
    View All» Brand Family: Chemicon View All» Storage Conditions: Precautions and Warnings: 1. The components of this kit are for Research Use only and are not intended for diagnostic procedures. 2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets. 3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube. View All» Detection Methods: Fluorescent View All» Product Name: Apo-Direct TUNEL Assay Kit View All» Materials Required but Not Delivered: 1. Flow Cytometer 2. Distilled Water 3. 1% (w/v) paraformaldehyde (methanol free) in PBS 4. 70% (v/v) ethanol 5. 37oC Water Bath 6. Ice Bucket 7. 12 x 75 mm flow cytometry test tubes 8. Micro-pipettor View All»
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