货号:AG854
品牌:Merck Millipore
规格:EA
目录价:¥4684.00
市场价格:¥3981.40
会员价格:¥3747.20
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Description:
Hepatitis B Virus, Core Antigen, recombinant
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Trade Name:
Chemicon (Millipore)
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Qty/Pk:
100 µg
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Product Overview:
C-terminal truncated Hepatitis B Virus core antigen (amino acids 1-156). The recombinant core antigen incorporates a C-terminal 6-HIS tag.
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Key Applications:
Positive Control
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Application Notes:
Recombinant HBcAg is a very large molecule with a cumulative molecular weight of 2.5 MegaDaltons and a particle size of 28 nm. To run an SDS-PAGE the sample much be completely denatured in SDS plus reducing agents prior to loading the sample on a gel. The most common difficulty in running gels with this material is having material that is not sufficiently denatured or reduced. Because of the aggregation of the protein, clean bands are not usually achieved, however one should see the expected dimer at 33kDa and a monomer of 19kDa with approximately 10% additional impurities if the samples are fully reduced. Some report that the use of 2M guanidium buffers help to reduce the aggregation as well.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Species:
Human
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Brand Family:
Chemicon
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Source:
Hepatitis B Virus
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Presentation:
Liquid containing 500mM NaCl, 50mM Tris-HCl, 1mM EDTA, pH 7.4, and 0.1% sodium azide.
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Storage Conditions:
Maintain at 2-8° C for short-term storage. Product may be stored at -70°C for up to 12 months from date of receipt.
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Product Name:
Hepatitis B Virus, Core Antigen, recombinant
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Alternate Names:
HBcAg
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Concentration:
0.88 mg/mL
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Purity:
90% pure, by SDS-PAGE and Western blot.
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