产品英文名称：Mouse IFN-γ ELISPOT Kit

产品中文名称：小鼠干扰素-γ Elispot 试剂盒

Mouse IFN-γ ELISPOT Kit 产品介绍

The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich enzyme-linked immunosorbent assay (ELISA), the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells,2,3 the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines.4,5,6 The sensitivity of the assay lends itself to measurement of even very low frequencies of analyte-producing cells (eg, 1/300,000).1 Recent developments in assay plate design and in ELISPOT plate-reader instrumentation have significantly improved the utility of the ELISPOT method for objective and rapid analysis of analyte-producing cells.

小鼠干扰素-γ Elispot 试剂盒的应用领域

• Transplantation

• Vaccine development

• Th1/Th2 analysis

• Autoimmunity

• Cancer

• Allergy

• Infectious disease

• Epitope mapping

• Humoral immunity

Mouse IFN-γ ELISPOT Kit 的成分

• Plates: 2 Pre-coated BD™ ELISPOT plates

• Biotinylated Detection Antibody

• Enzyme Conjugate (Streptavidin-HRP)

• Assay Diluent

• Wash Concentrate (20×)

• PBS (10×)

• AEC Substrate Buffer

• AEC Chromogen

• Certificate of Analysis, with lot-specific optimal dilution instructions

Mouse IFN-γ ELISPOT Kit 的优越性

•高灵敏度

•高通量，高含量的分析

•最小体积的生物材料的要求

•最小体积的生物材料的要求

•适用于冷冻/解冻的生物样品 •与其他检验方法兼容。例如，细胞分析。BD™ ELISPOT可以转移克隆，增殖实验，流式细胞术或其他分析方法。

Mouse IFN-γ ELISPOT Kit 使用注意事项

1. The Detection Antibody contains less than 0.1% sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

2. The Wash Concentrate contains 16% sodium chloride and is an irritant.

3. The AEC Substrate contains less than 10% dimethyl sulfoxide and less than 8% methanol and is harmful.

4. The AEC Chromogen contains 97.8% N,N-dimethylformamide and is toxic.

5. The enzyme conjugate contains 2.5% BSA. The source of all animal proteins is from USDA-inspected abattoirs located in the United States.