货号：AG56P

品牌：Merck Millipore

规格：100Ug

目录价：￥5368.00

市场价格：￥4562.80

会员价格：￥4294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Human Laminin (pepsinized) Purified Protein View All» Trade Name: Chemicon (Millipore) View All» Qty/Pk: 100 µg View All» Product Overview: AG56P contains a mixture of human laminins containing the beta1 chain, principally laminin 1, 2, 3, 6, 8 & 10. This preparation is immunologically and biologically identical to intact human laminin. AG56P is suitable for use in ELISA, production of antiserum, cell adhesion and attachment assays, and neurite-stimulation assays. This pepsinized laminin preparation runs as twin bands migrating at approximately 160kDa and 130kDa by SDS-PAGE under reducing conditions. Preparation: Chemicon's purified human laminin is prepared from freshly frozen human placenta tissue that is homogenized in PBS with a blender and the mixture centrifuged. The pellet is then washed with 0.5M acetic acid, collected and subjected to and extended Pepsin A digestion. The pepsin digest is neutralized, centrifuged to clear debris, and applied to an antibody column containing mouse anti-human laminin monoclonal 4E10 monoclonal antibody {MAB1921}. The solubilized laminin solution is passed over the column, and utlimately the laminin is released via acidification with KSCN. Then the material is dialyzed against PBS. The antibody 4E10 is specific for laminin beta1 chain. This chain is found in Laminin's 1,2,3,6,8,10 under the native conditions used to purify the material. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. View All» UniProt Summary: FUNCTION: SwissProt: P07942 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.SIZE: 1786 amino acids; 198066 Da SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-1 is a subunit of laminin-1 (EHS laminin), laminin-2 (merosin), and laminin-6 (K-laminin).SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.DOMAIN: SwissProt: P07942 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain. View All» Species: Human View All» Brand Family: Chemicon View All» Source: Human placenta, tested negative for hepatitis B virus, hepatitis C virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2, and Treponema pallidum. Handle as if potentially infectious. View All» Presentation: Purified laminin protein in liquid in PBS with 0.01% sodium azide. View All» Storage Conditions: Store at -20°C or -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a table-top centrifuge to dislodge any liquid in the container's cap. View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Gene Symbol:

• LAMB1

• MGC142015

• CLM
  View All» Product Name: Human Laminin (pepsinized) Purified Protein View All» Alternate Names: Laminin 1, 2, 3, 6, 8 & 10 View All» Concentration: 0.153mg/mL View All» Quality Assurance: Each lot is analyzed on SDS-PAGE for band integrity and expected sizes prior to packaging and release View All» UniProt Number: P07942 View All» Entrez Gene Number: NM_002291.1 View All» Purity: >/= 95% by SDS-PAGE. Purified from a mild pepsin digestion followed by affinity chromatography on monoclonal (4E10) anti-human laminin-Sepharose™. View All»
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