产品英文名称：SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric*

产品中文名称：SensoLyte® 490 HCV 蛋白酶荧光检测试剂盒

SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric* 产品介绍

丙型肝炎病毒（HCV）NS3／4A蛋白酶是病毒在NS3-NS4A，ns4a-ns4b，ns4b-ns5a，和ns5a-ns5b这些位点非结构蛋白裂解所需的。这些裂解的病毒蛋白的成熟至关重要。因此，该蛋白酶成为开发抗HCV药物的关键目标之一。该 SensoLyte®490 HCV蛋白酶检测试剂盒提供了方便的测定。HCV NS3/4A蛋白酶抑制剂和连续定量高通量筛选HCV NS3/4A蛋白酶活性采用5-FAM / QXL™490.荧光共振能量转移（FRET）肽。这个肽序列是来自NS4A /细胞裂解位点的半胱氨酸。自然裂解位点与γ-氨基丁酸取代（阿布）和易断裂的肽键以酯键。这些修改提高kcat / Km值的100倍以上，使检测的活性的NS3／4A在纳摩尔浓度的蛋白酶。在FRET肽，荧光淬灭的EDANS DABCYL。在分裂成两个单独的片段的HCV NS3/4A蛋白酶在阿布阿拉债券（见方案1），在荧光EDANS恢复，可以监测在340 nm的激发/发射= 490n实验是在一个方便的96孔或384孔微孔板格式进行。

SensoLyte® 490 HCV 蛋白酶荧光检测试剂盒的成分

Component A HCV NS3/4A protease substrate 250 μL

EDANS/DABCYL FRET peptide, Ex/Em=340

nm/490 nm upon cleavage

Component B EDANS, fluorescence reference standard 100 μM DMSO

Ex/Em=340 nm/490 nm solution, 10 μL

Component C 2X Assay buffer 25 mL

Component D Stop solution 15 mL

Component E DTT 1 M,1 mL

Component F Pep4AK HCV NS3 protease cofactor 150 μL, 600 μM

SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric* 的保存

• Store all kit components at -20°C

• Protect Components A and B from light

• Components C and D can be stored at room temperature for convenience

SensoLyte® 490 HCV 蛋白酶荧光检测试剂盒的英文描述

HCV protease is identified as an important drug-screening target. The SensoLyte® 490 HCV Protease Assay Kit uses an optimized FRET peptide substrate for the continuous measurement of HCV NS3/4a activity. This FRET substrate is cleaved specifically by HCV NS3/4a protease thereby liberating the C-terminal peptide-fluorophore fragment from the proximity quenching effect of the dark quencher, resulting in a more than 10-fold increase in fluorescence. This assay can detect HCV NS3/4a protease activity at 1 pmole. The kit contains: • EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nm upon cleavage) • Assay buffer • Fluorescence reference standard for calibration • A detailed protocol.

SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric* 系列产品订购信息

AnaSpec 61017-5 HCV NS3/4A protease genotype 1b, recombinant 5 µg

AnaSpec 61017-10 HCV NS3/4A protease genotype 1b, recombinant 10 µg

AnaSpec 72087 SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric* 1 kit

AnaSpec 71126 SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric* 1 kit

AnaSpec 72022-10 HCV NS3/4A protease mutant A156S, recombinant 10 ug

AnaSpec 72022-5 HCV NS3/4A protease mutant A156S, recombinant 5 ug

AnaSpec 72166-5 HCV NS3/4A protease genotype 1a, recombinant 5 µg

AnaSpec 72169-5 HCV NS3/4A protease genotype 4d, recombinant 5 µg

AnaSpec 72168-10 HCV NS3/4A protease genotype 3a, recombinant 10 µg

AnaSpec 72035-10 HCV NS3/4A protease mutant V170A, recombinant 10 µg

AnaSpec 72168-5 HCV NS3/4A protease genotype 3a, recombinant 5 µg

AnaSpec 72035-5 HCV NS3/4A protease mutant V170A, recombinant 5 µg

AnaSpec 72166-10 HCV NS3/4A protease genotype 1a, recombinant 10 µg

AnaSpec 72169-10 HCV NS3/4A protease genotype 4d, recombinant 10 µg

AnaSpec 72167-10 HCV NS3/4A protease genotype 2a, recombinant 10 µg

AnaSpec 72167-5 HCV NS3/4A protease genotype 2a, recombinant 5 µg

AnaSpec 71146 SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 1 kit

AnaSpec 71145 SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric* 1 kit

AnaSpec 72023-5 HCV NS3/4A protease mutant A156T, recombinant 5 ug

AnaSpec 72023-10 HCV NS3/4A protease mutant A156T, recombinant 10 ug