产品英文名称：Mouse Total IgG ELISA kit

产品中文名称：小鼠总 IgG 抗体 ELISA 试剂盒

Mouse Total IgG ELISA kit 的应用

本试剂盒是用于定量小鼠总的IgG抗体。本试剂盒使用的抗体对小鼠免疫球蛋白是特异性的并且不与来自其他物种的免疫球蛋白交叉反应。该试剂盒是用于研究和制造使用，不用于人类或动物的诊断应用。

Mouse Total IgG ELISA kit 的英文描述

The antibodies used in this kit are mouse immunoglobulin heavy and light chain specific. They do not cross react significantly with immunoglobulins from other species but they will cross react to varying degrees with other mouse immunoglobulins such as all IgG isotypes, IgA, and IgM. All of the Mouse Immunoglobulin ELISA kits can also be used to quantitate mouse immunoglobulin levels in serum of other animals administered mouse monoclonal antibodies in research studies.

Monoclonal antibodies are used in many applications such asdiagnostic or therapeutic agents as well as affinity supports for immunoaffinity purification of various products. These applications frequently require a sensitive assay to accurately quantitate the concentration of these antibodies in complex sample matrices. The antibodies used in this kit are mouse immunoglobulin heavy and light chain specific. They do not cross react significantly with immunoglobulin from other species but they will cross react to varying degrees with other mouse immunoglobulins such as all IgG isotypes, IgA, and IgM. The presence of high concentrations of mouse IgM and IgA immunoglobulin types in the sample can interfere in the ability of

this kit to quantitate mouse IgG. Cygnus Technologies provides kits for IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM immunoglobulins. See ordering information on the last page.

小鼠总 IgG 抗体 ELISA 试剂盒的使用步骤规则

The Mouse IgG assay is a two-site immunoenzymetric assay. Samples containing mouse IgG are reacted in microtiter strips coated with an affinity purified capture antibody. A second alkaline phosphatase enzyme labeled goat anti-mouse immmunoglobulin antibody is reacted, forming a sandwich

complex of solid phase antibody-mouse immunoglobulinenzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate p-nitrophenyl phosphate (PNPP) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly

proportional to the concentration of mouse IgG present. Accurate quantitation is achieved by comparing the signal of unknowns to IgG standards assayed at the same time.

Mouse Total IgG ELISA kit 的保存和稳定性

1 All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.

2 The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.

3 Reconstituted wash solution is stable until the expiration date of the kit.

小鼠总 IgG抗体ELISA试剂盒的使用注意事项

1 只用于研究或制造。

2 在用本试剂盒的试剂浓度，其他没有被认为是有害的。

3 本试剂盒只能由合格的技术人员使用。