产品英文名称：L.lactis HCP ELISA kit

产品中文名称：L.lactis HCP ELISA ELISA 试剂盒

L.lactis HCP ELISA kit 的应用

该试剂盒已专门开发和验证用来检定在乳酸乳球菌中表达的重组产品中乳酸乳球菌HCPS污染物的存在。该试剂盒利用采自乳酸乳球菌介质HCPs的抗体和亲和性纯化。该试剂盒是用于研究和制造使用，不用于人类或动物的诊断应用。

L.lactis HCP ELISA 试剂盒的英文描述

This kit has been specifically developed and validated for use in detecting L.lactis HCPs as contaminants in products produced by recombinant expression in Lactococcus lactis. The antibodies used in this kit were generated against and affinity purified from HCPs obtained from the spent culture media of a null cell line. Our assay validation has determined that the LOD for this kit is ~0.125ng/mL.

L.lactis HCP ELISA kit 的使用步骤规则

The L. lactis assay is a two-site immunoenzymetric assay.Samples containing L. lactis HCPs are reacted with a horseradish peroxidase (HRP) enzyme labeled anti-L. lactis antibody simultaneously in microtiter strips coated with an affinity purified capture anti-L. lactis antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody.The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of L. lactis HCPs present.

L.lactis HCP ELISA 试剂盒的保存和稳定性

* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.

* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.

* Reconstituted wash solution is stable until the expiration date of the kit.

L.lactis HCP ELISA kit 的使用注意事项

*只用于研究或制造。

*停止反应试剂是0.5N H2SO4。避免接触眼睛，皮肤，和服装。在用本试剂盒的试剂浓度，其他没有被认为是有害的。

*本试剂盒只能由合格的技术人员使用。