货号：ABT38

品牌：Merck Millipore

规格：

目录价：￥3978.00

市场价格：￥3381.30

会员价格：￥3182.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MMP-8 (Hinge Region) Antibody | ABT38 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the hinge region of MMP-8. View All» Molecular Weight: ~58 kDa observed.When used against the reduced protein, the antibody may identify bands at 85 kDa (proform) or a series of further cleaved active forms, including 64 kDa, 58 kDa and others. This will depend on cell conditions and cell type used. MMP-8 levels in quiescent cells and tissues are minimal, and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types vary greatly in the amount of MMP-8 produced. View All» Epitope: Hinge Region View All» Immunogen: KLH-conjugated linear peptide corresponding to the hinge region of human MMP-8. View All» Background Information: Matrix metalloproteinase 8 (MMP-8 or neutrophil collagenase), first described in neutrophils, is a classical type-I collagenase. Like MMP-1, MMP-8 is able to cleave intact type-I collagen at the same site in all three strands. MMP-8 is heavily glycosylated, and the inactive enzyme precursor proform or zymogen has a mass of ~85 kDa. The zymogen is quickly activated to the 64 kDa form, and this breaks down to a cascade of active forms, such as the 58 kDa form and others. MMP-8 is not produced by most cell types; it has been found in PMNL neutrophils, arthritic chondrocytes and gingival fibroblasts. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. View All» Application Notes: Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected MMP-8 in normal human spleen tissue.Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated MMP-8 from NIH/3T3 RIPA lysate. View All» Control: HL-60 conditioned media View All» Quality Assurance: Evaluated by Western Blot in HL-60 cell lysate supplemented with a conditioned media.Western Blot Analysis: 1 µg/mL of this antibody detected MMP-8 in 10 µg of HL-60 conditioned media. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P22894 View All» Entrez Gene Number: NP_002415 View All» Gene Symbol:
  • MMP8

  • PMNL-CL

  • MMP-8

  • HNC

  • CLG1
    View All» Alternate Names:
    • Neutrophil Collagenase

    • Matrix Metalloproteinase 8
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation
        View All» Entrez Gene Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Can degrade fibrillar type I, II, and III collagens.COFACTOR: Binds 3 calcium ions per subunit. & Binds 2 zinc ions per subunit.SIZE: 467 amino acids; 53412 Da SUBCELLULAR LOCATION: Cytoplasmic granule. Note=Stored in intracellular granules.TISSUE SPECIFICITY: Neutrophils.DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains. View All» Product Name: Anti-MMP-8 (Hinge Region) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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