货号：ABT28

品牌：Merck Millipore

规格：100Ug

目录价：￥5513.00

市场价格：￥4686.05

会员价格：￥4410.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Autotaxin Antibody | ABT28 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Autotaxin at the substrate binding region. View All» Molecular Weight: ~100/125 kDa observed. 3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa. The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates. View All» Epitope: Substrate binding region View All» Immunogen: KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region. View All» Background Information: Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Canine

• Bovine
  View All» Species Reactivity Note: Demonstrated to react with Human. Predicted to react with Mouse, Rat, Canine, and Bovine based on 100% sequence homology. View All» Application Notes: Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues. View All» Control: Oligodendrocyte precursor cell lysate View All» Quality Assurance: Evaluated by Western Blot in oligodendrocyte precursor cell lysate.Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q13822 View All» Entrez Gene Number: NP_001035181 View All» Gene Symbol:
  • ENPP2

  • ATX

  • PDNP2
    View All» Alternate Names:
    • Ectonucleotide pyrophosphatase/phosphodiesterase family member 2

    • E-NPP 2

    • Autotaxin

    • Extracellular lysophospholipase D

    • LysoPLD
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]. View All» UniProt Summary: FUNCTION: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.CATALYTIC ACTIVITY: 1-alkyl-sn-glycero-3-phosphoethanolamine + H2O = 1-alkyl-sn-glycerol 3-phosphate + ethanolamine. COFACTOR: Binds 2 zinc ions per subunit (By similarity).Binds 1 calcium ion per subunit (By similarity).ENZYME REGULATION: Inhibited by lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P). Inhibited by EDTA and EGTA Probable. SUBCELLULAR LOCATION: Secreted. Note: Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells. TISSUE SPECIFICITY: Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. INDUCTION: Up-regulated in massively obese subjects with glucose intolerance, and during adipogenesis. PTM: N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity (By similarity).It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SEQUENCE SIMILARITIESl Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.Contains 2 SMB (somatomedin-B) domains.BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:KM=0.5 mM for 16:0-LPC (at pH 8.5) KM=5.5 mM for pNP-TMP (at pH 8.5)KM=11.3 mM for pNppp (isoform 1)KM=5.7 mM for pNppp (isoform 2)KM=19.8 mM for pNppp (isoform 3)Vmax=1.9 nmol/min/µg enzyme with pNppp as substrate (isoform 1)Vmax=0.67 nmol/min/µg enzyme with pNppp as substrate (isoform 2)Vmax=1.6 nmol/min/µg enzyme with pNppp as substrate (isoform 3)pH dependence:Optimum pH is 9.0 (isoform 1), 8.0 (isoform 3). Isoform 1 is less sensitive to pH. Isoform 1, isoform 2 and isoform 3 all retain some activity at pH 9.5.Temperature dependence:Isoform 1 and isoform 3 are active from 45 to 60 degrees Celsius. View All» Product Name: Anti-Autotaxin View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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