货号：ABT118

品牌：Merck Millipore

规格：EA

目录价：￥3588.00

市场价格：￥3049.80

会员价格：￥2870.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Shugoshin-like 1 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~80 kDa observed. Seven isoforms are known to exist. Based upon the immunogen sequence, this antibody is predicted to react with isoform 1 (64 kDa), isoform 6 (60 kDa), and isoform 7 (25 kDa). This protein has been observed at ~80 kDa (Karamysheva, Z. et al. (2009). J Biol Chem. 284(3):1772-1780.; McGuinness, B. E. et al. (2005). PLoS Biol. 3(3):e86.). An uncharacterized band may be observed at ~140 kDa in some cell lysates. View All» Epitope: 0 View All» Immunogen: KLH-conjugated linear peptide corresponding to human Shugoshin-like 1. View All» Background Information: Shugoshin-like 1 (SGOL1) prevents premature dissociation of the cohesin complex from centromeres after prophase, thereby protecting centromeric sister-chromatic cohesion in early mitosis. SGOL1 has also been shown to promote stable kinetochore-microtubule attachment and sense tension across sister kinetochores. While SGOL1 peaks at mitosis, it is normally degraded upon exiting mitosis. SGOL1 contains two degradation motifs, KEN box and destruction box, which are targeted for ubiquitination by anaphase-promoting complex/cyclosomes (APC/C). When bound to Cdh1, APC/C ubiquitinates mitotic regulators such as SGOL1, cyclin a, cyclin b, and securin for destruction. APC/C is regulated by the spindle checkpoint mechanism, which prevents the activation of APC/C and destruction of its substrates in response to kinetochores that have not properly attached to the mitotic spindle. In prophase, mitotic kinases such as Plk1 phosphorylate cohesin. During the metaphase-to-anaphase transition, separase cleaves cohesin to allow for sister-chromatid separation. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Rat, and Mouse. View All» Application Notes: Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Shugoshin-like 1 in HeLa cells during prophase and metaphase of the cell cycle. View All» Control: PC3 cell lysate View All» Quality Assurance: Evaluated by Western Blot in PC-3 cell lysate.Western Blot Analysis: 2.5 µg/mL of this antibody detected Shugoshin-like 1 in 10 µg of PC3 cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q5FBB7 View All» Entrez Gene Number: NP_001012409 View All» Gene Symbol:
  • SGOL1

  • SGO1
    View All» Alternate Names:
    • Shugoshin-like 1

    • hSgo1

    • Serologically defined breast cancer antigen NY-BR-85
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» Product Name: Anti-Shugoshin-like 1 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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