产品英文名称：Human MSC Analysis Kit

产品中文名称：人类间充质干细胞分析试剂盒

流式细胞仪是一个描述，分析，和分离干细胞及其衍生物的功能强大的方法。干细胞生物学的新发现有的重要性有放大的多潜能干细胞治疗和改善研究院对正常疾病过程的理解。然而，区分文化固有的异构性仍然是干细胞研究的主要挑战。

BD Human MSC Analysis Kit 的物质组成

hMSC positive cocktail (CD73, CD90, CD105); hMSC negative cocktail (CD11b, CD19, CD34, CD45, HLA-DR); compensation controls and isotype controls.

BD 人类间充质干细胞分析试剂盒的组分

PE hMSC Negative Cocktail 50 Tests,20 μl

PE hMSC Isotype Control Negative Cocktail 50 Test,20 μl

hMSC Positive Cocktail 50 Test,20 μl

hMSC Isotype Control Positive Cocktail 50 Test,20 μl

FITC Mouse Anti-Human CD90 50 Test,5 μl

PE Mouse Anti-Human CD44 100 Test ,5 μl

PE Mouse IgG2b, k Isotype Control 50 Test,5 μl

APC Mouse Anti-Human CD73 50 Test,5 μl

PerCP-Cy™5.5 Mouse Anti-Human CD105 50 Tes,t5 μl

BD Human MSC Analysis Kit 的英文描述

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues. MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes. MSCs also have immunomodulatory effects in vivo and in vitro. In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow. Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR. MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271. In this kit we include the panel that was proposed by the ISCT. In addition, we designed this kit to be modular so that additional markers could be “dropped-in”. Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5 CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PE

CD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibody conjugates. Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocol to analyze multiple samples. Individual positive markers are included for compensation set-up