货号：ABS71

品牌：Merck Millipore

规格：

目录价：￥3433.00

市场价格：￥2918.05

会员价格：￥2746.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GEF-H1 Antibody | ABS71 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes GEF-H1. View All» Molecular Weight: ~ 120 kDa observed View All» Immunogen: KLH-conjugated linear peptide corresponding to human GEF-H1. View All» Background Information: Guanine nucleotide exchange factor H1 (GEF-H1) is a guanine nucleotide exchange factor containing 3 characteristic domains; a DH (DBL-homology) domain that functions to interact with and promote GTP loading on RhoA, a pleckstrin-homology (PH) domain that participates in the binding and targeting of microtubules to tight junctions, and a phorbol-ester/DAG-type zinc finger. GEF-H1 functions to promote the GDP to GTP exchange for the activation of Rho-GTPases. Various studies have suggested that GEF-H1 may have several diverse roles such as; dendritic spine motility, cell motility and polarization, cell cycle regulation, antigen presentation, epithelial barrier permeability, leukemic cell differentiation, cancer, and accelerated cell proliferation within tumor cells. Currently, there are three known isoforms resulting from alternative splicing. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. View All» Application Notes: Western Blot Analysis: 2 µg/mL of this antibody was shown to detect GEF-H1 on 10 µg of L6 cell lysate. View All» Control: HEK293 cell lysate View All» Quality Assurance: Evaluated by Western Blot in HEK293 cell lysate.Western Blot Analysis: 2 µg/mL of this antibody detected GEF-H1 on 10 µg of HEK293 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q92974 View All» Entrez Gene Number: NP_004714 View All» Gene Symbol:
  • GEF

  • GEF-H1

  • LFP40

  • P40

  • GEFH1
    View All» Alternate Names:
    • Rho/Rac guanine nucleotide exchange factor (GEF) 2

    • Rho/Rac guanine nucleotide exchange factor 2

    • Proliferating cell nucleolar antigen p40

    • Microtubule-regulated Rho-GEF

    • Guanine nucleotide exchange factor H1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq]. View All» UniProt Summary: FUNCTION: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in Ref.1 (Ren Y., et al. (1998). J. Biol. Chem. 273:34954-34960.). May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. SUBUNIT STRUCTURE: Interacts with 14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA/STK6 and MAPK1. Interacts with RHOA and RAC1.SUBCELLULAR LOCATION: Cytoplasm. Cell junction › tight junction (By similarity). Golgi apparatus. Cytoplasm › cytoskeleton › spindle. Note: Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. DOMAIN: The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.PTM: Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. SEQUENCE SIMLARITIES: Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.SEQUENCE CAUTION: The sequence AAC97383.1 differs from that shown. Reason: Sequence differs at a large extent from the sequence shown in the paper.The sequence AAH20567.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA33634.1 differs from that shown. Reason: Frameshift at positions 887 and 984. View All» Product Name: Anti-GEF-H1 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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