货号：ABD52

品牌：Merck Millipore

规格：

目录价：￥4264.00

市场价格：￥3624.40

会员价格：￥3411.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-BAF57 Antibody | ABD52 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes BAF57 at the HMG box region. View All» Molecular Weight: ~50 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 47 and 42 kDa. A doublet is observed at ~50/51 kDa. View All» Epitope: HMG box region View All» Immunogen: KLH-conjugated linear peptide corresponding to human BAF57 at the HMG box region. View All» Background Information: BAF57, also known as SMARCE1, is a core subunit of the SWI/SNF complex, which is required for transcriptional activation of genes normally repressed by chromatin. The SWI/SNF chromatin remodeling complex is critical for androgen receptor transcriptional and the BAF57 subunit facilitates direct interaction with the receptor. Studies suggest a blockade of the androgen receptor-BAF57 interaction may target agonisty-induced androgen receptor function. Data also suggest that the SWI/SNF complex is required for transcriptional activation by the estrogen receptor. ChIP assays demonstrated BAF57 is recruited to the estrogen-response promoter, pS2. View All» Species Reactivity:

• Human

• Rat

• Mouse

• Horse

• Rhesus Macaque

• Bovine
  View All» Species Reactivity Note: Demonstrated to react with Human and Rat. Predicted to react with Mouse, Equine, Rhesus Macaque, and Bovine based on 100% sequence homology. View All» Application Notes: Western Blot Analysis: 0.5 µg/mL from a representative lot detected BAF57 on 10 µg of L6 cell lysate.Immunohistochemistry Analysis: 1:300 dilution from a representative lot detected BAF57 in anal squamous cell carcinoma and ductal carcinoma tissue. View All» Control: Jurkat cell lysate View All» Quality Assurance: Evaluated by Western Blot in Jurkat cell lysate.Western Blot Analysis: 0.5 µg/mL of this antibody detected BAF57 on 10 µg of Jurkat cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q969G3 View All» Entrez Gene Number: NP_003070 View All» Gene Symbol:
  • SMARCE1

  • BAF57
    View All» Alternate Names:
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1

    • BRG1-associated factor 57

    • BAF57
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. SUBUNIT STRUCTURE: Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B), and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1 (SNF5/INI1). Other subunits specific to each of the complexes may also be present. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Also binds to the SRC/p160 family of histone acetyltransferases (HATs) composed of NCOA1, NCOA2, and NCOA3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with RCOR1/CoREST, NR3C1 and ZMIM2/ZIMP7. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin (By similarity). SUBCELLULAR LOCATION: Nucleus DOMAIN: The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development (By similarity).SEQUENCE SIMILARITIES: Contains 1 HMG box DNA-binding domain. View All» Product Name: Anti-BAF57 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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