货号：ABC132

品牌：Merck Millipore

规格：

目录价：￥4264.00

市场价格：￥3624.40

会员价格：￥3411.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Rb (Ser807/Ser811) Antibody | ABC132 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Rb phosphorylated at Ser807/Ser811. View All» Molecular Weight: ~110 kDa observed View All» Epitope: Phosphorylated Ser807/Ser811 View All» Immunogen: KLH-conjugated linear peptide corresponding to human Rb phosphorylated at Ser807/Ser811. View All» Modifications:

• Phosphorylation

• Phosphorylation
  View All» Background Information: The retinoblastoma (Rb) gene product (also known as p105, p110 or pRb) is a tumor suppressor protein that is missing or mutated in a variety of human tumors. In the hereditary childhood form of retinoblastoma, Rb is lost from chromosome 13. Rb regulates cell proliferation and differentiation by controlling progression through the restriction point within the G1 phase of the cell cycle. Rb forms complexes with many proteins, and this binding activity is required for growth suppression. Rb specifically interacts with the E2F family of transcription factors to repress E2F-mediated transcription. Rb also interacts and alters the activity of other transcription factors including Elf-1, ATF-2, MyoD, BRG-1, c-myc, N-myc. Rb recruits a histone deacetylase, HDAC1, to E2F-1 to repress transcription. Two Rb related proteins p107 and p130 also function to regulate specific members of the E2F transcription factor family. View All» Species Reactivity:
  • Human

  • Mouse
    View All» Species Reactivity Note: Demonstrated to react with Human. Predicted to react with Mouse based on 100% sequence homology. (11/12 amino acids identical in Rat and chicken Rb). View All» Application Notes: Peptide Inhibition Assay Analysis: 0.05 µg/mL from a representative lot peptide blocked on MOLT4 cells stimulated with PMA. View All» Control: Untreated and lambda phosphatase-treated MOLT4 cell lysates stimulated with PMA View All» Quality Assurance: Evaluated by Western Blot in untreated and lambda phosphatase-treated MOLT4 cell lysates stimulated with PMA.Western Blot Analysis: 0.05 µg/mL of this antibody detected Rb on 10 µg of untreated and lambda phosphatase-treated MOLT4 cell lysates stimulated with PMA. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P06400 View All» Entrez Gene Number: NP_000312 View All» Gene Symbol: RB1 View All» Alternate Names:
    • Retinoblastoma-associated protein

    • p105-Rb

    • pRb

    • Rb

    • pp110
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Cell Function Assay
        View All» Entrez Gene Summary: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.SUBUNIT STRUCTURE: Interacts with ATAD5. Interacts with PRMT2 (By similarity). The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor. Interacts with heterodimeric E2F/DP transcription factor complexes containing TFDP1 and either E2F1, E2F3, E2F4 or E2F5, or TFDP2 and E2F4. The unphosphorylated form interacts with EID1, ARID3B, KDM5A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with NDC80. Interacts with GRIP1 and UBR4. Interacts with ARID4A and KDM5B. Interacts with E4F1 and LIMD1. Interacts with SMARCA4/BRG1 AND HDAC1 (By similarity). Interacts with adenovirus E1A protein, HPV E7 protein and SV40 large T antigen. Interacts with PSMA3 and USP4. Interacts (when methylated at Lys-860) with L3MBTL1. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in the retina.DOMAIN: The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.PTM: Phosphorylated in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylated at Lys-860 by SMYD2, promoting interaction with L3MBTL1. INVOLVEMENT IN DISEASE: Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ('cat eye') is investigated. Defects in RB1 are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.Defects in RB1 are a cause of osteogenic sarcoma (OSRC) [MIM:259500].SEQUENCE SIMILARITIES: Belongs to the retinoblastoma protein (RB) family. View All» Product Name: Anti-phospho-Rb (Ser807/Ser811) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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