货号：AB962

品牌：Merck Millipore

规格：

目录价：￥3615.00

市场价格：￥3072.75

会员价格：￥2892.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Substance P Antibody, pain | AB962 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum has been evaluated for its ability to stain nerve fibers in the external median eminence of the chicken and opossum using both the immunoperoxidase and immunofluorescence procedures. View All» Immunogen: Synthetic substance P bound to keyhole limpet hemocyanin (KLH) with carbodiimide. View All» Species Reactivity:

• Chicken

• Opossum
  View All» Application Notes: TISSUE EVALUATION: Formalin-fixed and paraffin-embedded sections (6 microns thick) of median eminence tissue were prepared from the domestic chicken and opossum. Cryostat sections of the above tissues can also be used in the staining procedure. IMMUNOPEROXIDASE STAINING: 1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4). 2. The antiserum diluted 1:500 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes. 3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these two steps excess reagent is washed from the section as described before. 4. The final step in the staining involved treating section with a mixture of 3,3'-diamino-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted. IMMUNOFLUORESCENCE STAINING: 1. Same as Step 1 above. 2. The tissue is reacted with the antiserum, diluted 1:20 in (PBS) for 30 minutes at 37*C. 3. After washing off the excess antiserum with PBS, the section is treated with fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope. COMMENTS: The antiserum described here has been established to give a positive staining of the nerve fibers in the medium eminence of two species using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time, e.g. 24 hours. View All» Presentation: Liquid antiserum. Contains 0.1% sodium azide. View All» Storage Conditions: Store at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P20366 View All» Entrez Gene Number:
  • NM_003182.1

  • NM_013996.1

  • NM_013997.1

  • NM_013998.1
    View All» Gene Symbol:
    • TAC1

    • NKNA

    • Hs.2563

    • NK2

    • PPT

    • NKA

    • TAC2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry (Paraffin) View All» Entrez Gene Summary: This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P20366 # Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.SIZE: 129 amino acids; 15003 Da SUBCELLULAR LOCATION: Secreted.SIMILARITY: SwissProt: P20366 ## Belongs to the tachykinin family. View All» Brand Family: Chemicon View All» Product Name: Anti-Substance P Antibody, pain | AB962 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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