货号：AB6013

品牌：Merck Millipore

规格：EA

目录价：￥3696.00

市场价格：￥3141.60

会员价格：￥2956.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MMP-13 Antibody, hinge region | AB6013 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes MMP-13 at the hinge region. View All» Molecular Weight: ~60 kDa observed View All» Epitope: Hinge Region View All» Immunogen: KLH-conjugated linear peptide corresponding to the hinge region of human MMP-13. View All» Background Information: MMP13 (matrix metallopeptidase 13 or collagenase 3) is a member of the matrix metalloproteinases (MMPs). MMPs are Zn2+- and Ca2+-dependent endopeptidases which function in the turnover of extracellular matrix components [Matrisian, 1992]. Main subfamilies of MMP are collagenases, gelatinases, stromelysins and membrane-type matrix metalloproteinases [Nagase, 1997]. Three homologous collagenases have been identified in human tissues: Interstitial collagenase, neutrophil collagenase and collagenase-3. Collagenase-3 hydrolyzes type II collagen 5- to 6- times faster than type I and type III collagens. The enzyme also exhibits high activity towards gelatin and it degrades SERPINS as a1-antichymotrypsin and plasminogen activator inhibitor-2 [Knauper et al., 1996]. Collagenase-3 is inhibited in a 1:1 stoichiometric fashion by TIMP-1, TIMP-2 and TIMP-3. Collagenase-3 is expressed during fetal bone development [Stahlebackdahl, 1997]. In adult human tissues collagenase-3 has been detected only in pathological conditions: in malignant tumors [Freije et al., 1994], in chronic ulcers [Valaamo et al., 1997], in arthritic cartilage [Mitchell et al., 1996] and synovium [Wernicke et al., 1996]. View All» Species Reactivity:

• Human

• Primate

• Rhesus Macaque

• Horse
  View All» Species Reactivity Note: Demonstrated to react with human. Predicted to react with primate, rhesus macaque, and equine based on 100% sequence homology. Other homologies: Rat and mouse (93% sequence homology). View All» Application Notes: Western Blot (SNAP i.d.) Analysis: 5 µg/mL from a previous lot detected MMP-13 in 10 µg of MCF-7 cell lysate.Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected MMP-13 in rectal carcinoma tissue. View All» Control: MCF-7 cell lysate View All» Quality Assurance: Evaluated by Western Blot in MCF-7 cell lysate.Western Blot Analysis: 0.5 µg/mL of this antibody detected MMP-13 in 10 µg of MCF-7 cell lysate. View All» Purification Method: Protein A Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P45452 View All» Entrez Gene Number: NP_002418 View All» Gene Symbol:
  • MMP13

  • MANDP1

  • CLG1

  • MMP13

  • MMP-13
    View All» Alternate Names:
    • matrix metallopeptidase 13 (collagenase 3)

    • collagenase 3

    • matrix metalloproteinase 13 (collagenase 3)

    • matrix metalloproteinase 13

    • Matrix metalloproteinase-13
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.SIZE: 471 amino acids; 53820 Da TISSUE SPECIFICITY: Seems to be specific to breast carcinomas.DOMAIN: SwissProt: P45452 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.DISEASE: SwissProt: P45452 # Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common.SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains. View All» Product Name: Anti-MMP-13, hinge region View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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