货号：AB5885

品牌：Merck Millipore

规格：EA

目录价：￥4050.00

市场价格：￥3442.50

会员价格：￥3240.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Histamine Antibody | AB5885 View All» Replaces: AB134 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Histamine. No reactivity to L-histadine containing peptides. Preabsorption of the antiserum with a histamine-BSA or histamine-ovalbumin conjugate (1-100 mg/mL depending on tissue) completely abolished the specific reaction. SPECIES REACTIVITIES: Vertebrates and invertebrates (human, rat, mouse, frog, fish, mollusks, flatworm). View All» Immunogen: Histamine conjugated to KLH with EDAC. View All» Species Reactivity: Rat View All» Application Notes: Immunohistoschemistry on brain and stomach using PAP or ABC: 1:1,000-1:4,000. Suggested fixative is 4% EDAC (1-ethyl-3(3-dimethylaminoproply)-carbodiimide) or EDAC + PFA. Immunocytochemistry on cultured cells (2H3 mastocytoma): 1:100-1:2,000. This antibody is not suitable for use on samples fixed only with aldehydes. Optimal working dilutions must be determined by end user. SUGGESTED IMMUNOCHEMISTRY PROTOCOLS FOR AB5885 Immunohistochemistry for vertebrates The histamine antibody can be applied to different species and tissues. The serum gives good results only when used on properly fixed tissue sections. Specific detection of histamine-like immunoreactivity requires the use of EDAC (1-ethyl-3(3-dimethylaminoproply)-carbodiimide) fixative. Immersion fixation is suitable for small animals and tissue blocks. Perfusion is recommended for vertebrates. Solutions 1. 0.1 M sodium phosphate buffer, pH 7.4 2. 4% EDAC (1-ethyl-3(3-dimethylaminoproply)-carbodiimide) in 0.1M sodium phosphate buffer (pH 7.4); the final pH will be around 7.0. Always use freshly prepared solution. 3. 0.9% saline Application to small laboratory animals (vertebrates) 1. Open the thorax under suitable deep anesthesia (e.g. pentobarbital). Open the right heart auricle with small scissors, Perfuse through the left ventricle or ascending aorta with saline until all blood is washed out (about 50 mL in a rat). 2. Perfuse with fresh 4% EDAC through the same needle. Use about 1 mL/gram of body weight. 3. Remove the tissues and immerse them in 4% EDAC for 2 hours or overnight. 4. Wash the tissue in 0.1 M sodium phosphate buffer containing 20-30% sucrose overnight. 5. Freeze the tissues and make cryostat sections for immunohistochemistry. Tissues from many species can be fixed by immersion in 4% EDAC overnight at 2-8°C. The pieces should be no thicker than 0.5 cm because the fixative does not pentrate very well. Note that the tissues are not firm hard after fixation with EDAC. Handling free floating sections requires experience, sections collected on adhesive-coated slides are easy to handle. If aldehyde fixation is necessary for e.g double staining procedures, EDAC perfusion should be followed by perfusion with 4% paraformaldehyde in the same buffer (and if needed 0.5% glutaraldehyde + 0.2% saturated picric acid). However these samples must be treated with 0.5% borohydride after sectioning. Incubation with the histamine antiserum (diluted in PBS with 0.25% Triton) is best performed at 2-8°C for 24-48 hours. Extended incubation times of up to 5 days have also been used successfully. Immunocytochemistry for cell culture The histamine antibody can be applied to primary cell cultures and cell lines from different species and tissues. The serum gives good results only when used on properly fixed tissue sections. Specific detection of histaminelike immunoreactivity requires the use of EDAC (1-ethyl-3(3-dimethylaminoproply)-carbodiimide) fixative. Immersion fixation is suitable for small animals and tissue blocks. Perfusion is recommended for vertebrates. Solutions 1. 0.1 M sodium phosphate buffer, pH 7.4 2. 4% EDAC (1-ethyl-3(3-dimethylaminoproply)-carbodiimide) in 0.1M sodium phosphate buffer (pH 7.4); the final pH will be around 7.0. Always use freshly prepared solution. 3. 0.9% saline Incubation procedure 1. Pour out the culture medium and wash the cells with 0.9% saline or 0.1 M sodium phosphate buffer. (This can be omitted if the cells are sensitive to changes.) 2. Pour in freshly prepared 4% EDAC, fix at 2-8°C for 0.5-12 hours depending on the thickness of the culture. 3. Wash with 0.1 M sodium phosphate buffer or PBS 2 x 10 min. 4. Preincubate with normal serum from the species used for production of the secondary antiserum for 20 minutes at room temperature. Pour out the normal serum, do not wash. 5. Incubate with the histamine antiserum diluted appropriately (1:200-1:5,000) for 24-48 hours at 2-8°C. If electron microscopy or double staining procedures with other antibodies are needed, the cells can be fixed after 4% EDAC with a mixture of 4% paraformaldehyde, 0.5% glutaraldehyde and 0.2% saturated picric acid for 30 minutes. After thorough washing, these samples must be treated with 0.5% sodium borohydride (and washed extensively) before incubation. Any routinely used detection method (indirect immunofluorescence, PAP procedure, biotin-streptavidin peroxidase) can be used. View All» Presentation: Rabbit antisera. Liquid containing 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunocytochemistry

• Immunohistochemistry
  View All» Brand Family: Chemicon View All» Product Name: Anti-Histamine Antibody | AB5885 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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