产品英文名称：C-peptide ELISA Kit

产品中文名称：C-肽ELISA检测试剂盒

§ 检测原理：ALPCO C-肽ELISA是一个夹层式免疫检测试剂盒。96孔微孔板涂有一层C-肽特异性单克隆抗体。将标准物，对照品和样品一并添加到微型板小孔中与检测缓冲液混合。然后将微孔板放在摇床上室温温育1小时，700－900转/分钟。第一次温育结束，用清洗缓冲液清洗小孔并烘干。添加结合物（配制方法参照说明书），微孔板放在摇床上第二次室温温育1小时，700－900转/分钟。添加TMB作用底物，放在微孔板摇床上室温温育15分钟，700－900转/分钟。第三次温育结束，添加反应终止液，调节分光光度计到450nm测定OD值。由于发光强度与样品中C-肽含量成比例，因此可以根据OD值确定C-肽含量。

§ 检测过程：各添加25μl标准物，对照物和样品→加50µl检测缓冲液→微孔板摇床室温温育1小时，700－900rpm→清洗缓冲液清洗6次→加100µl结合物（配制方法参照说明书）→微孔板摇床室温温育1小时，700－900rpm→清洗缓冲液清洗6次→添加100µl TMB作用底物→微孔板摇床室温温育15分钟，700－900rpm→添加100µl反应终止液→分光光度计450nm读数

ALPCO C-peptide ELISA kit英文简述：

The ALPCO C-peptide ELISA kit is a FDA registered in vitro diagnostic tool for the quantification of human C-peptide in a clinical setting or research laboratory. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, a single C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components of the C-Peptide ELISA and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.

INTENDED USE：The ALPCO C-peptide ELISA is designed for the quantitative determination of C-peptide in human serum and plasma.

PRINCIPLE OF THE ASSAY：The ALPCO C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the Assay Buffer. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The Conjugate is then added, and the microplate is incubated a second time on a microplate shaker at 700-900 rpm, washed, and blotted dry. TMB Substrate is added, and the microplate is incubated a third time at room temperature on a microplate shaker at 700-900 rpm. Once the third incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.