产品英文名称：R-spondin-1 Fc fusion, human

产品中文名称：人重组R-spondin-1 Fc fusion蛋白

R-spondin-1 Fc fusion, human产品介绍：

R-spondin-1是权威WNT信号通路的天然增强剂。当与激活β-catenin信号通路的WNT蛋白联合使用时，R-spondin-1能通过与LGR5和LGR4融合而有效提高权威WNT蛋白的活性。

向小鼠注射重组R-spondin-1蛋白可以激活β-catenin信号通路和肠道下皮细胞的增殖，为临床改善化疗诱导结肠炎操作提供了基础和依据。

本产品是全片段R-spondin-1与人IgG1细胞Fc片段的C-终端相融合而得到的。这种融合保证了体内外蛋白的稳定性而不影响其生物活性。StemRD公司的R-spondin-1 Fc融合蛋白是由人293细胞作为分泌蛋白，并经过一系列的色谱分析法纯化而得到。

R-spondin-1 Fc fusion, human产品英文简述：

R-spondin-1 is a natural enhancer of the canonical WNT pathway. When used together with WNT proteins that activate the beta-catenin pathway, Rspondin- 1 enhances the activity of canonical WNT proteins by binding to LGR5 and LGR4 (refs.) Injection of recombinant R-Spondin-1 into mouse causes activation of the-catenin pathway and proliferation of intestinal crypt cells, which forms the basis for a clinical trial in amelioration of chemotherapy-induced colitis.

This product is the full-length R-Spondin-1 fused at its C-terminus to the Fc domain of human IgG1. This fusion increases the stability of the protein in vitro and in vivo without compromising its biological activity. StemRD’s R-Spondin-1 Fc fusion protein is produced in human 293 cells as a secreted protein and purified by protein A chromatography.

• Purity: Greater than 90% as determined by SDS-PAGE analysis

• Biological Activity: The activity was determined by using a TCF reporter gene assay in cultured

human cells. The EC50 ranges from 5 - 20 ng/ml in the presence of 10ng/mL human WNT-3a. Activity in other assays should be determined by each individual setting.

• For Research Use Only. Not for Use in Human