产品英文名称：Dkk-1 Fc fusion, human

产品中文名称：人重组Dkk-1 Fc fusion蛋白

Dkk-1 Fc fusion, human产品介绍：

DKK-1是重组人Dickkopf相关蛋白1的简称，是DKK蛋白家族（DKK1-4）的一员。DKK最初被认为是非洲爪蟾蜍的前期成形细胞。通过机理研究发现DKK-1通过与共同受体LRP5/6一起形成抑制复合物，可以有效抑制Wnt/β-catenin信号通路。研究人员发现敲除DKK-1基因小鼠的脑部无法形成，从而证实Wnt/β-catenin信号通路的抑制在脊椎动物的后模式与前模式发展中必不可少。

当DKK-1融于人IgG1蛋白的Fc片段时，这种融合避免了蛋白在体内体外环境下的显著失活，有效保证了其稳定性。

Dkk-1 Fc fusion, human英文简述：

DKK-1, Dickkopf-related protein-1, is a member of the DKK protein family which includes DKK-1 through DDK-4. DKK was originally identified as a head-forming molecule in Xenopus. Mechanistic studies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway by forming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling is essential for posterior patterning and anterior development in vertebrates, supported by the discovery that DKK-1 knockout mice lack head formation. When DKK-1 is fused to the Fc fragment of human IgG1. This fusion renders stability of the protein in vitro and in vivo without significant loss of activity.

StemRD expresses DKK-1 Fc fusion in human 293 cells and purifies it by protein A column.

• Purity Greater than 90% as determined by SDS-PAGE analysis

• Biological Activity The activity was determined by using a TCF reporter gene assay in cultured

human cells. The IC50 ranges from 200 - 1000 ng/ml in the inhibition of 100 ng/mL WNT-3a activity. Activity in other assays should be determined by each individual setting.

• For Research Use Only. Not for Use in Human