货号：AB5154-50UL

品牌：Merck Millipore

规格：50UL

目录价：￥5027.00

市场价格：￥4272.95

会员价格：￥4021.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cav2.2 Antibody | AB5154-50UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Cav2.2 (alpha 1B subunit (both LMW and HMW forms) of voltage-gated calcium channel, Cacna1b). Does not cross react with any other calcium channel antigens tested so far. SPECIES REACTIVITIES: Rat. It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested. View All» Immunogen: Purified peptide from amino acids 851-867 of rat Cav2.2 (Accession Q02294). View All» Species Reactivity: Rat View All» Application Notes: In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each). Western blot: 1:100-1:200 using ECL on rat brain membranes. Immunohisto/cytochemistry on rat brain sections. Immunoprecipitation Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. View All» Control: CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at 2-8°C in undiluted aliquots for up to 2 weeks. For longer periods, it is recommended to add glycerol (ACS grade or better) at a ratio of 1:1 and store the antibody at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.. View All» UniProt Number: Q00975 View All» Entrez Gene Number: NM_000718.1 View All» Gene Symbol:

• CACNA1B

• BIII

• CACH5

• Cav2.2

• CACNN

• CACNL1A5
  View All» Alternate Names: Voltage Gated Calcium Channel (N-type) alpha 1B Subunit; Cacna1b View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Immunocytochemistry

  • Immunoprecipitation

  • Western Blotting
    View All» Entrez Gene Summary: Voltage-dependent Ca(2+) channels are multisubunit complexes found in the membrane of many excitable cells that regulate calcium entry (see MIM 601011). N-type calcium channels, which control neurotransmitter release from neurons, are dihydropyridine-insensitive and omega-conotoxin-sensitive. The alpha-1 subunit forms the pore through which calcium enters the cell, and is encoded by a family of at least 5 genes.[supplied by OMIM] View All» UniProt Summary: FUNCTION: SwissProt: Q00975 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the high-voltage activated (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin- IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.SIZE: 2339 amino acids; 262496 Da SUBUNIT: Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMS1 and RIMBP2 (By similarity).SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Isoform Alpha-1b-1 and isoform Alpha-1b-2 are expressed in the central nervous system, but not in skeletal muscle or aorta.DOMAIN: SwissProt: Q00975 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.PTM: Phosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK (By similarity).SIMILARITY: Belongs to the calcium channel alpha-1 subunit family. & Contains 1 EF-hand domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Cav2.2 Antibody | AB5154-50UL View All» Concentration: 0.75 mg/mL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://16ao.com/thread-153838.htm
    © Merck Millipore代理AB5154-50UL Anti-Cav2.2 Antibody;抗-Cav2.2抗体store at -20℃，产品报价联系微信jinshanbio